LOCUS       Exported     3635 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3635
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1672..1719
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3567..3583
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3584..3615
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1555..1574
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1540
                     /note=""
                     /translation="MTLNIEDEHRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRIQHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKTGTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDNSRLI*"
                     /label="Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII)"
     primer_bind     complement(1724..1740)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1807..2395)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2586..3446)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3447..3551)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1575..1634
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GACCCTGAAC ATTGAAGATG AGCATCGTCT
       61 GCATGAAACC TCTAAGGAGC CAGATGTATC ACTGGGTTCC ACCTGGCTGT CTGATTTCCC
      121 GCAGGCGTGG GCGGAAACCG GCGGCATGGG CCTGGCGGTA CGTCAAGCAC CGCTGATCAT
      181 CCCGCTGAAG GCCACCTCTA CCCCGGTTAG CATTAAACAG TACCCGATGT CTCAGGAAGC
      241 GCGCCTGGGC ATTAAACCGC ACATCCAGCG TCTGTTGGAT CAGGGTATCC TGGTGCCGTG
      301 CCAGAGCCCG TGGAACACCC CTCTGCTGCC GGTAAAAAAA CCGGGTACCA ACGATTACCG
      361 TCCGGTTCAG GACCTGAGAG AAGTTAACAA ACGCGTGGAA GATATTCATC CGACCGTGCC
      421 GAACCCGTAC AACCTGCTTT CCGGTCTGCC GCCGAGCCAC CAGTGGTATA CCGTGCTGGA
      481 TCTGAAAGAT GCCTTCTTCT GCCTGCGCCT GCACCCGACC TCCCAGCCGC TGTTCGCGTT
      541 CGAGTGGCGC GACCCTGAAA TGGGCATCTC TGGCCAACTG ACTTGGACCC GTTTGCCTCA
      601 GGGTTTCAAA AATTCTCCGA CCCTGTTCGA TGAAGCACTG CATCGTGACC TGGCGGATTT
      661 TCGCATTCAG CATCCGGACC TGATCCTGCT GCAATATGTA GACGATCTGC TGCTGGCAGC
      721 CACGTCTGAG CTGGATTGTC AGCAGGGCAC GCGTGCGCTG CTGCAAACCC TGGGCAACCT
      781 GGGCTACCGC GCCTCTGCTA AAAAGGCGCA GATTTGCCAG AAACAGGTTA AATACCTGGG
      841 TTACCTGCTG AAAGAAGGCC AGCGTTGGCT GACGGAAGCG CGCAAAGAAA CCGTAATGGG
      901 TCAGCCAACT CCGAAAACCC CGCGTCAGCT GCGTGAGTTC CTGGGTACCG CTGGCTTCTG
      961 CCGTCTGTGG ATTCCGGGCT TCGCCGAAAT GGCGGCGCCG CTGTATCCAC TGACCAAAAC
     1021 CGGCACTCTG TTCAACTGGG GCCCGGATCA GCAGAAAGCC TACCAGGAGA TTAAACAGGC
     1081 ACTGCTGACC GCGCCGGCAC TTGGTCTGCC GGACCTGACG AAACCATTCG AACTTTTCGT
     1141 TGACGAGAAA CAGGGTTACG CTAAAGGTGT TCTTACTCAG AAATTGGGTC CGTGGCGTCG
     1201 CCCGGTAGCG TACCTGAGCA AAAAACTGGA CCCGGTGGCT GCTGGTTGGC CGCCGTGCCT
     1261 TCGTATGGTT GCGGCCATTG CTGTTCTGAC TAAAGATGCG GGCAAACTGA CAATGGGTCA
     1321 GCCGCTGGTG ATCCTGGCGC CGCACGCTGT AGAAGCGCTG GTGAAACAGC CTCCGGATCG
     1381 TTGGCTGAGC AATGCTCGCA TGACTCACTA CCAAGCGCTG CTGCTGGACA CCGATCGTGT
     1441 GCAGTTCGGC CCGGTTGTTG CACTGAATCC GGCGACTTTG CTGCCACTGC CGGAGGAAGG
     1501 ACTGCAACAC AACTGTCTCG ACAACTCCCG TCTGATTTGA AGAGCTTTGA GACCAGTATT
     1561 AGGCGTCAAG GTCCCCCGTG ACTTACTTCT ACCGATAGGG ATAACAGGGT AATCCTTCCC
     1621 CATAAGTAGC ATTCCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     1681 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     1741 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     1801 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1861 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1921 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1981 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     2041 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     2101 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     2161 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     2221 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     2281 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     2341 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG
     2401 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     2461 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     2521 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     2581 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     2641 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     2701 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     2761 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     2821 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     2881 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     2941 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     3001 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     3061 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     3121 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     3181 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     3241 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     3301 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     3361 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     3421 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     3481 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     3541 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC
     3601 CGGAGGCTTT TGACTTGGTT CAGGTGGAGT GGGAG
//