LOCUS       Exported     3866 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3866
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1903..1950
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3798..3814
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3815..3846
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1771
                     /label="Eco31I"
                     /note=""
     primer_bind     complement(1955..1971)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2038..2626)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2817..3677)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3678..3782)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1806..1865
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GGAGAGTCTG CCAAAGATCG CCGTCACCCC
       61 GAACAATGAT AAGGCTGCAT CACATTCAAA AGACGAAAAT TTAGCTACCA ACACCATTAA
      121 GAAGAACGTC AAACCGAAAT ACGGTGATGC GGCCTTCCTG GAATACACCC GTCTTATCGT
      181 TAACCATCCG AATTATTTTG GCATGCCAGA CCCGTTTGGC GAGAAAGGTG AAATTCAGTG
      241 GGAGGCACCG TCCAACCGTG CCTCAGGGAA GTTTAAACAT ACGCATCAAC GTCGGTACGA
      301 GTGGTGGAAG AATAAAGCGC GCAGTATTGG AATTGACCCG GATACCGAGA AAGCTTGGAT
      361 CAGTAAAACC GCAAAACTGA TTCATCCACT CGGGGTGAAA CCTTGCAAGA AATGCGGCAA
      421 AGAGATGGAA TTATCTTATT CATATCCTAA TGAACATTTC TTTTCTCGTG TACGCAAACT
      481 GAATTATATC GATGAAACTT TCGAATTAAG CCAGAACGAG CACATCGTTG ATCTATTGAC
      541 TCGCCTGGAT GACCGTTTTG GCGAACGGAT TTATTTAGAC TTGCCGCACC TGTTTTCTAC
      601 GAAAAGTATT ACTATTCCTG ATATTAGCTC GAACCTGGAA GCATGGATTG AATATCTGAA
      661 AGAACAGTAT ATCCCACAGG AAAGCCGGAT GTTGTCACCG GGGGCCATGG CCAACCCGCC
      721 CGACCGTTTC GATGGCTTTC ATTCCTTTAA CCGTTGCTGT CGCTCTATTG CGGACAAAGG
      781 TAGAACTAAA GAGAACTTGA AATCATACGT CACAGATCGC CGTGTGTTCG AATACTGGGT
      841 CGATGGCGAT TGGGTAGCCG CGGATCGTTT AATGGGTCAG GTGCGCACCA ATAATATTTT
      901 CATCAACGAA GAATGCCTGA ACGCGGGTAA CGGTGGCTTG CATCCGACGC CGTGTCAAGC
      961 TGATCACATA GGCCCCATTT CCCTCGGCTT TTCGCACCGC CCCCAGTTTC AACTGCTGTG
     1021 TAAAAGCTGC AATTCGGCCA AAAATAATCG GATGTATCTG TCCGATATAA TTAGCCTGCT
     1081 TGAAGCTGAG AATGAGGGAC ACACAGTGAT TAGCTGGTTT GCGGAGGAAG TGTGGAACCG
     1141 ACTGAAACAT TCGGTGGACG ATAGCGAAAA AGCGCTGCGC CTGAGCAAAA TTCTACGTGA
     1201 TAATCGCCAT ACCTATATGA ACCTGTTGAA AAAGATCATG GACGAGGGCT ACTACACATT
     1261 CCTGGCAAGT CTGCTGCACC TGGAAGTTGC CAACTATAAC CCTATTTTCG AAGGATTGTG
     1321 TATCTCCAAT CACCTGACAC ACTACAAAAG TCTGAAAAAA ATCAAGCGTG AAAGCAAATA
     1381 TGCAGCGGTA CAGAAAACGC GTCGCATTCG CATCGCGTTT ACCAGCCTCA ATGATTATCA
     1441 TAGGAAAGAG AACCGTAACG CGTTTATTGT TAGCAATGAA CTTTCTGAAA AATTCTTCTC
     1501 CGAAGCAATG GATAACTTAA AAAGTCTGTC GGAAATTACG AGCTGCTTAG ATGAAAAGAT
     1561 CAGTGGTATC ATCTCTGAAA ATAGCGATTC AAAAAATGAG TTTCGCACCA TAATTACGGA
     1621 CCTGAGAGAA ATCGTTACGA ACAATAAAGA AAAATTCAAT CTTATACTCA AGTACCTTAT
     1681 TTCTGGGATG TCGGAAATCG GTAAAGAACT CGAATCGTAT TGGGAAAACG ACCGATACGT
     1741 GCGCTCCATC CCGGAAGAGT TTATCGAGTG AAGAGCTTTG AGACCGCCAT TGGAAAACTG
     1801 AACGTTGTAA ATCAATAGTG CAATGCTAGG GATAACAGGG TAATTAAGCG TGATAGGGCA
     1861 CAACGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1921 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1981 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     2041 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     2101 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     2161 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     2221 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     2281 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     2341 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     2401 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     2461 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     2521 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     2581 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     2641 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     2701 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     2761 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     2821 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     2881 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     2941 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     3001 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     3061 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     3121 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     3181 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     3241 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     3301 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     3361 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     3421 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     3481 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     3541 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     3601 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     3661 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     3721 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     3781 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     3841 TTGACTTGGT TCAGGTGGAG TGGGAG
//