LOCUS Exported 3764 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3764 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1801..1848 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3696..3712 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3713..3744 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1669 /label="Eco31IA" /note="" primer_bind complement(1853..1869) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1936..2524) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2715..3575) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3576..3680) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1704..1763 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GGAAGAAATT TTTTACATGA AACATATTCA 61 TTTAATTAAT AGCCTGTCGT TAGACGAAAC CACTAAATTT ACTAAGAAAG CCACCGGGAA 121 ATATTACACT GATCCTAAGA TCGCCCTCCT GATGATCGAA AAACTGTTGC CGCTGATTAA 181 CAGCTGCGAT AAAAAAAGTT ATAATGTGGC AGACCCGTTT AGTGGCGATG GCCGTCTTAT 241 CACGCTGCTG ATTAAGCAGT GGATGATTAA TGGTTTCCCG GATGTCGAAT GGAACGTCTA 301 CCTGTTTGAC ATTGAGAACA CCGGTTTGAC CTATGCGAAA AACGCGTTGT CCGAGTTGAA 361 ACTGGCGGGC GCGAATATTA ACATCACGAT CAAGAATAGC GATGTGTTCT ATGAATTTAA 421 AAAATATGTT GACTACTTCG ATTGTGTCAT AACCAACCCG CCCTGGGAAA ACATTAAACC 481 TGATAGCCGC GAACTGGATT TTTTTGAGCC GTCGATGAAA AGCATGTATA TTGATAGTTT 541 GCGCGAGTTC GACGACTATT TAAGTCGCGT CCTGCCGTAC AGCCAGCCGA AACGTAAATT 601 CGCGGGCTGG GGCACAAACC TGAGCCGCGT TGGTGCGGAA TTGTCACTGG AAATCTGCAA 661 TAAAAATGGT CTAGTAGCCA TTGTGATGCC AGCAAGTTTT TTCGCAGATG AACAGTCTTA 721 CATTCTACGT GAGAAGTTTT TTAACTCTGG ACGAATTGAT TGTATTAACT ATTACCCGGC 781 AGAAGCTAAA CTGTTCGGCG GTGCTGATGT TAGCTCTTGC TCTTTTATCT TCAACAAAGG 841 CGAAAGCCTG AATGACAATA TCCAGCTGAG CGTATATGAT AAAAATCTGA ACATCAAGTC 901 CCTAGGGTTC TTTGACCTCT CCTCTATCGA CTCCCAATAT TTGTCGATTC CAGTCTCACA 961 AGGTGTTCAT GCCGTGCACC TGTTACGGAA ACTTCAAGAA GGTTATCCAA CCTGGGGGTC 1021 CCTGGAGAAA AACGGCGAGA TTTGGGCAGG GCGTGAAATT GATGAAACAG GAAGCAGTGA 1081 TTGGACGCAG AAATCGGGTG GTGGCCTTCT TTTTATTAAA GGCAAGATGA TCGGACGTTA 1141 CAATTTCCAT AATGAGAAAT CATTAAGGAT CACCAAAAAG ATCGACAAAG TGTTATCGAA 1201 TAGTAACTTT GTGCGAATCG CTTGGCGCGA TATATCACGG CCGTCACAGA AAAGACGCAT 1261 GATTGCCACG ATAATTCCCC CAAACAGCCT GGCCGGCAAT AGCCTGGGGG TTGTGTACTA 1321 TAAATCTGGA AGTCAAGATT CCCTCTTCTC GCTGCTCGGT ATCATTAACT CACTCTGCTT 1381 TGAATTTCAG CTGCGTTCTT TCCTGGCAAC AGGCCATGTG TCGCTGTCAG CCCTGCGTAA 1441 AACGGCGATC CCTTCGGAGA AAATACTGCT TCAACACAGC GAGTTAAAGC AGCTTGTAAT 1501 CAGTTGTATC GAAGGTTGTT GCGATGCGGA GCTTAAGATT GAAGCGTATG TTGCTAAAAA 1561 CATTTACAAG CTGGATCTCA ATGAATTTAA TAAACTGCTG TCCAGCTTCG ATAAAATTGA 1621 ATTAGCGGAA AAAGAATCCT TGCTGCGCAT CTTTCAGCAC TATGATTGAA GAGCTTTGAG 1681 ACCGGGTGCC TTGCTAATCT AGTGCCAAGA CGCGCTTCTG ACAGTAGGGA TAACAGGGTA 1741 ATCCCCACTG ATGTGAGCGA GGACGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA 1801 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC 1861 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA 1921 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 1981 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 2041 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 2101 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 2161 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 2221 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 2281 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 2341 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 2401 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 2461 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 2521 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 2581 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 2641 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 2701 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 2761 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 2821 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT 2881 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 2941 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 3001 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 3061 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 3121 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 3181 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 3241 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 3301 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 3361 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 3421 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 3481 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 3541 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 3601 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 3661 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA 3721 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAG //