LOCUS       Exported     3314 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3314
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1351..1398
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3246..3262
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3263..3294
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1219
                     /label="Eco31IB"
                     /note=""
     primer_bind     complement(1403..1419)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1486..2074)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2265..3125)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3126..3230)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1254..1313
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GACTAAATCT GAGACATTCA TGATTCCAAA
       61 CCACAAAGCG GCGAAGCTGA GCGAATTAGA TATGATGATT GTGAACTCTG TTCCGCCGGG
      121 TGGTAATTGG AAAAACATCC CTCTGGACGT CCCGTCAAAA CGTATTGAAC AAATACGAGA
      181 TAGTTATGCT CAGGGAAAAG GCAGCCGCAG CACTTATTAT GGCAGACTCC TCCCCGATAT
      241 GCCCGCTTAC ACCATTAATA CGTATTTTAA CCGCCCCGGC AACGGCTGCC ATATCCATTA
      301 CGAACAGGAT CGTGTACTGA GCCAGCGTGA AGCGGCCCGG CTTCAAAGCT TCCCTGATGA
      361 CTTCATCTTT TTCGGTGGCC AGACAGCGAT TAACACCCAG ATCGGCAATG CTGTACCGCC
      421 GTTTCTGGCA TTTCTGATAG CAAAAGAGAT TGAAAAAGCC ATCGGCAACA CGGGCTATTA
      481 TATTGACCTG TTTTCCGGTG CAGGGGGTTT AGGTCTGGGA TTTAAGTGGG CCGGCTGGAC
      541 CCCGTTACTG GCGAACGATA TCGAGGAGAA ATACCTTCAA ACGTATTCAA ATAACGTGCA
      601 TAAGGAGGTG CTGTGTGGGA GTATCAGTGA CAACGAAACC TTTTCCAAAA TCGCGGATAA
      661 AATTTCTGGG TTTAAAAAAT TGTACTTCGA CAAGCAACTA TGGATTCTGG GCGGTCCACC
      721 GTGCCAGGGT TTTTCGACTG CGGGGAATGC CCGTACGATG GACGATCCTC GTAATTCGCT
      781 GTTCATGCAT TACAAGAGTC TCTTGAACGA AATCAAACCG AATGGCTTCA TTTTCGAAAA
      841 TGTTGCAGGA TTGTTAAATA TGGAAAAAGG TAAAGTTTTT GAGCGCGTGA AAGAAGAATT
      901 TTCGTCCACG ATGAAAACCA TGAACGGCTG GATACTGAAC TCGGAACACT ACGCCATTCC
      961 GCAGCGCCGC AAACGTGTGA TTCTAGTCGG AAGCAATGAT CCATTGTTTT CAATCGAACC
     1021 GCCGCAGAAG CTGACCGAGG ATAAAGAGTC ATGGGTCAGT GTTAAAGATG CACTGTCTGA
     1081 TCTTCCACCT CTTCAACACG GGGAAGATGG TAGCGGCAAA TATTACATTC ATCACCCAGA
     1141 AAATGACTAT CAGCTGTTCA TGCGGGGTAA CATTACCCCG TCCGAATACT ATGAACGCAA
     1201 TATCAAGCCG AGCCTGTGAA GAGCTTTGAG ACCGGCCTTT TATTGGGTCC AGTCCGCAAA
     1261 CCCTGATCTT ATTTTAGGGA TAACAGGGTA ATGTACAAAT AGGGTCGAAA TTCCGCTGAG
     1321 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1381 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1441 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1501 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1561 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1621 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1681 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1741 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1801 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1861 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1921 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1981 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2041 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2101 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2161 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2221 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2281 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2341 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2401 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2461 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2521 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2581 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2641 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2701 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2761 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2821 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2881 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2941 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     3001 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3061 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3121 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3181 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3241 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC
     3301 AGGTGGAGTG GGAG
//