LOCUS       Exported     3422 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3422
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1459..1506
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3354..3370
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3371..3402
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1327
                     /label="SapI"
                     /note=""
     primer_bind     complement(1511..1527)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1594..2182)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2373..3233)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3234..3338)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1362..1421
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GCGCCGTCTG GCTACTCAAC GTCGTGAAGA
       61 TGCATACAAG AGCAACCGCG ATTATCAGAC CGTACATGAA GCACAGTCTC TGCGCGTAAA
      121 CTCGACCGAC GACGACAACT TGTCTCTGTT CCTGTTAAAA GATATCTCTC CTCGTGAAGA
      181 TTCGAAAAAC ATTGTTGGCT TTGGCGGCTT CGTTAAACCG GAAATCGCCA CCACCATGGC
      241 GCTGACTCTG ACTACCGACA TTGATAAGCA AATCAAAAGC GTACCGCTGT CCAGCAACTG
      301 GAACCGCATC AGCATCGTTG CGAAATTCGC GTCTAACCCT AGCGTGTCCA TCACCCTGGG
      361 CTTCGATCAG ACCCCGTGGG TAGATTTCTG GGGCATCAAT TCCGATGACA TCGGTCTGTC
      421 TTTCGTGTCG GACGCGGTTC CGCTGGAAAT GTCAATGATC GATAGCATCC ATATCGCGCC
      481 GGAAACCCTG TACTTAGACC ACAGCAGCGC ATGCCTGCTG GACATCGATC CGGTTGAATC
      541 CACCCGCTTT AAAACGGGTC ACGGCGACCC GCTGTCTCTG AAAAAATGCA GCTACTGCGG
      601 CCGTCTGCTG CCGATCGATC TGGAACGTCC GGGTAAACTG TCCTTCCACA AACATCGTGC
      661 GAAAATTACT AACCATCAGA ACGAGTGTCG CTCCTGCAAA AAGTGGCGTA TCAACAACTC
      721 TTTTAACCCG ATGCGCACCA TTGACCAGCT GAACGAATCC GCGCTGATCA CCCGTGAACG
      781 TAAAATCTTC CTCCAGGAAC CGGAAATCCT GCAAGAAATC AAAGACCGTA CCGGTGCCGG
      841 TCTGAAATCC CAGGTTTGGG AACGTTTCCA CCGCAAATGC TTCAACTGCC GTAAAGACCT
      901 GAAACTGTCT GAAGTACAAC TGGACCATAC TCGTCCGCTG GCGTATCTGT GGCCGATCGA
      961 TGAACACGCA ACCTGCCTGT GTGCCCAGTG TAACAACACT AAAAAAGACC GCTTTCCGGT
     1021 GGACTTCTAT AGCGAACAGC AGATCCGTGA ATTGTCTGAC ATCTGTGGTC TGCCGTACCA
     1081 GGACCTGTGC GCACGTTCTC TGAACCTGGA TCAACTGGAC CGTATCGAAC GCAACATTGC
     1141 GGAGTTCTCT AAGGAATGGG ACGTACGTAC CTTCGCTTCC ACCGCGCGAC GCATTTCTGA
     1201 AGTTTATCCT GCTCGTGACC TGTTCGAAAC GCTGAAAAAA GAATCAGAAA GCGCATACAA
     1261 CAAAATCATC GAAAAACTGA AAGAACGTCC GGATGCATTA CTGGACGAAG CTCTGCCGCT
     1321 GGATTGAAGA GCTTTGAGAC CCAGTCCTTG CCAATCGAAG TACCCATCTA GCGGCTGCGG
     1381 CATAGGGATA ACAGGGTAAT GGCATGCCCA TCTTACCAAC TCGCTGAGGT GTCAATCGTC
     1441 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1501 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1561 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2161 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2221 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2281 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2341 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2401 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2461 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2521 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2581 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     2641 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2701 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2761 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2821 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2881 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2941 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3001 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3061 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3121 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3181 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3241 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3301 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3361 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG
     3421 AG
//