LOCUS       Exported     3254 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3254
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1291..1338
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3186..3202
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3203..3234
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1159
                     /note=""
                     /label="M1.SapI"
     primer_bind     complement(1343..1359)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1426..2014)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2205..3065)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3066..3170)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1194..1253
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GCTGGAAACC CTGGAGCTGG TTAACAAAAT
       61 CGCGGAATTT CAGCGTAAAC TGCCGTATAC CCAAGACGAC TATAAATCTC GCAGCTGGGG
      121 TCACCCCCTG CACAGCCTGT GTAGCTATCA GGGCAAGCTG AAACCGAGCC TGGCTCACTG
      181 GCTGGTTAAA ACCTTCTCTC CGGAAGGTGG TACCGTTCTG GACCCAATGG GCGGTGTTGG
      241 CACGATCGCG TTCGAAGCAG CACTGACCGG CCGTGTAGGT ATCACCAACG ATAAATCCCC
      301 ACTGGCTGCA ACCGTCACCG CGGGTAAACT CGCGCCGTCC TCCATCCTGG AAGCTGAAGA
      361 AGCGATCGGT CGTCTGGCGG AAGATATTGA AAGCGTGGAC CTGTCTGCTG CTGATTATGA
      421 AGCCGCGAAC TTCGGTCTGA ACGCACGCGT GTCTGACTAT TACCATCCGG ATACTCTGAA
      481 AGAAATCCTC CGTGCTCGTC GTATTTTCTC TGAACGCCGT GAAGCTTACC CGGCCTTTGT
      541 TTGGGCATCT CTGCTTCATG TGCTGCACGG CAACCGCCCA TACGCGCTGT CTCGCATCAG
      601 CCATCCGATC ACCCCGTTCA ATCCGTCTGG TGTTGCTGAA TACCGTAGCG TGGTGGAAAA
      661 AATCGCGCAC CGCGCGCGCC TGGCTCTGCG TAACCCGCTG CCGGAAGCTT TCACCTCTGG
      721 CGCAGCCATC GAAGGCGACT TTCGTGACCT GTCGGAACAT ATCAACGAGC CGGTGGATGC
      781 TATTATCACT AGCCCGCCGT TCATGGGCAT GCGTTTCGAT CGCCCGAACT GGCTGCGCCT
      841 GTGGTTCTGC GGCTGGGATG CAGAAGATTT CTGGACCACC TCTCTAGGCT TCCTTGAACG
      901 TCATCAGGTG AAAAGCCGTG ACTCCTACAT CGACTTCTTT GAAATGTCCA TCAAAACGCT
      961 GAAGCAGGAT GGTCTGCTGG TTATGCACCT GGGCTCCGGT GGTAAAAAAA ATCTGGTGAA
     1021 CGACCTGAAA TCGCTGGCCG TGCCGCTGTT CGAACTGGCG GGCGAGGTCA TCGAGGACGT
     1081 TGACGATCAC CAAACGCACG GTATTCGTGA TCGCGGTCTG ACCACAAAAC ACCACCTGCT
     1141 GTTCTTCAAA CCGGCCTGAA GAGCTTTGAG ACCCCCCGTG AATGCTATGT TCTCGTCCCT
     1201 ACGAGTTGAT ATGATAGGGA TAACAGGGTA ATAAAAACCT ATGTCCCTCT ATGCGCTGAG
     1261 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1321 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1381 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1441 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1501 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1561 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1621 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1681 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1741 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1801 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1861 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1921 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1981 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2041 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2101 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2161 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2221 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2281 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2341 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2401 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2461 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2521 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2581 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2641 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2701 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2761 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2821 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2881 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     2941 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3001 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3061 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3121 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3181 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC
     3241 AGGTGGAGTG GGAG
//