LOCUS Exported 3425 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3425 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1462..1509 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3357..3373 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3374..3405 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1330 /label="M2.SapI" /note="" primer_bind complement(1514..1530) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1597..2185) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2376..3236) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(3237..3341) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1365..1424 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GTCTGTGGAC GCTCCATCCC CGGCGCGCCG 61 CCGCGGCCAG GCAGCAACTT CCGGTCGTGG CACCAATGAG AACCGCCTCC CGATCGACCT 121 GGGCGTTACC TTCCGTGACA ACCGCAACCG TCCGGTCCAT TCTTGGTACC CGTACGTTGA 181 GGGTTTCAGC GCGGCATATG TTGAAGGCGT GCTGGCCCCG TACAACGGTC ACAACGTGGC 241 GGTTTACGAT CCGTTCGGTG GTTCTGGAAC TCTTCAGAGC ACTGCGTCCT GGCTGGGTAT 301 CAACTCCTTC TACAGCGAAG TGAACCCGTT CATGCGCTTC GTCGCTGAAG CGAAAGTCAA 361 CGCAACCCTG AAAGCAGCGC AAAACAAAGA CGTATTCCGC TGCGCCGCGA AAGAGTTCCT 421 GGACATGCTG TCTGAAAAAG AACTGGCTCA CCGTGGCCGT AGCGTCGATC TGTCCCAGTA 481 CTACTCCGCG TTCCCGGGTC GTGACTTCTT CGAAGAAGAA CACATCCGCC AGCTGCTCGC 541 GGCGTGTGAC GCCGCCCGTC TGATTGGCTC CGATTACGCT TGGGTTCGCC AGCTGCTGCT 601 GCTGGCATGC GCAGCCAACG CAGTTCATTC GAGCAACATG ACCCGTCGTG CGGACCTGCG 661 TCGTCGCCGC CAGAATGAAT ATATCAATCG TAAAGTTGAC GTTGCGCGTT TCATCTCCGA 721 CACTGTTCAG GCTATGCTGG ATGACGTGGA ACAAGTTCCG TTCGGCGCAG TTGCGTCCCA 781 TTATGTCTCC GATGACTGTC GTGATCTGCC TAGCCGCTAC ATCGACTGTT TCGATATCGC 841 TATCACTTCT CCGCCGTACC TCAACGGCAC CAACTATTTC CGCAACACCA AAATTGAACT 901 GTGGCTGCTG GGTTTTCTGA GCCACGAAAG TGAACTGCCG AAATTCTGCC GTGAGGCGAT 961 CACTGCCGGC ATCAATAACG TGTCTGGGAA CAAAGCTCTG GACCACCACT TTGATGTTGT 1021 CGAAGATGTG GCGACTAAAC TGGACGACGT CGCTCCGGAC CGCCGCATCC CGAAACTCGT 1081 ACGTCACTAC TTTTCTGATA TGTATGAAGT TCTGACCAGT GTTCGTAGCA GCCTGCGCCT 1141 GGGCGGTCGC TTCATCCTGG ATATCGGCGA CTCTAAGTTC TATGGTGTGC ACGTGCCGGT 1201 TGATCGTATC CTGGTAGAAC TGGGTAAGCA GGTGGGCTTC CAGCTGCACC AGGATGTTGT 1261 TATCGCCCGT CGTCACTCTC GTGACAAAAC CCCCTTGGTG CAAGTGGAAC TGGAATTTCG 1321 TAAGGCCTGA AGAGCTTTGA GACCCCAGCC TCAGATTGTT GTCTGACCTA GAATAGGAGA 1381 TCTGTTAGGG ATAACAGGGT AATCCACACC GCTAGAGCAC GCTACGCTGA GGTGTCAATC 1441 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG 1501 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC 1561 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA 1621 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 1681 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 1741 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 1801 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 1861 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 1921 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 1981 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 2041 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 2101 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 2161 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC 2221 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 2281 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 2341 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 2401 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 2461 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA 2521 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 2581 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 2641 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 2701 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 2761 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 2821 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 2881 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 2941 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 3001 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 3061 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 3121 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 3181 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 3241 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 3301 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA 3361 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT 3421 GGGAG //