LOCUS Exported 3395 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3395 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 1432..1479 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3327..3343 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3344..3375 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1300 /label="M.XbaI" /note="" primer_bind complement(1484..1500) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1567..2155) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2346..3206) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3207..3311) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1335..1394 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GAAAAACGCG ACCACCCAGG CGCGCGCGGT 61 ACAGGGTGAT TGCCTGGTCG AGCTGGCGCG CCTCGAAGAA TGTGAAGCTG ACCTGGTATA 121 CCTTGACCCG CCGTTTTTTA CCAATCGTCG CCACTCTTCT ATCTCTCGTG ATCGTATCAA 181 TGCGTTTTCG TTTGATGATG CTTGGGGCGA CCTGGGGGAA TATGCGGACT TCATGGAGCA 241 GCGTCTGCGC GAAGCTCACC GTGTCCTGAA ACACTCGGGT TCTATCTTCG TCCACTGCGA 301 TACCTCAGCC AACTTCCTGT TACGTACCCT GCTGAACAAC ATCTTTGGTG CCAGCCAGTT 361 TCGTTCCGAA ATCATCTGGT CCTATAAACG TTGGAGCAAC AGCGCTCGCA ACCTGCTCCC 421 GGCTCACCAG ACTATCTTGT TCTACAGCAA ATCAGATGCG TATAAATTCA ACGTTCTGCA 481 CGGTTCCTAC TCTGAAACTA CTAACGTTGA TCAGATTCTG CAACTGCGTC AGCGTGATGC 541 TGACGGCGTT TCCAAATACG CTACCGACAA GCTGGGTAAC ACCATCTACG GCACCGAAAA 601 GAACGGCGTT CCCTTGAACG ACGTTTGGGC GATCCCGTTC CTGAACCCGA AAGCGAAAGA 661 ACGCACTGGC TATCCGACTC AGAAACCGAT TCTGCTGCTG GAGCGCATCA TCGAAATCTC 721 TACTGACCCG GGCGACTTTA TCGTTGATCC GTTCTGCGGG TCTGGCACCA CTCTGGTAGC 781 TGCGGCAATC CTGGGCCGTC GCGCTTTCGG TATCGATACT AGCCGCGAAG CTGTAGCGCT 841 CGCAAACCGC CGTATCGAGG AACCGGTGCG TACCGACTCT GCCCTGCTGA AAAAAGGCCG 901 TCTGGCTTAC GCGACTGCAA ACCTGAAAGC CCTGGCAGTA CTGGAAGGTC TGGATTTCGT 961 TCCGGTTCAC CGCAACTCCG GCATCGACGC ATTTCTGCGC AGCCCGGAGG GTGAGCCGCT 1021 GGTTCCGGTT CGCGTTCAGC GCAACGGCGA ATCGATCTCT GATGCCGCGG CGTCCCTGGC 1081 GCGTGCAGCC ATCTCTAAGA AAGCGAAGAA CGCAATTCTG GTGCGTACCA ACGAAGAAAC 1141 GGGCCTGTTC TCGACTGACG CATTGCCGCC GTTCATGCAT CTGATCGAAT CTACCGCCCT 1201 GCGCATCAAA TCCTCACTGG ACGCGTCTTT GATCGCGGGC GAAACCAAGA GCGCTGCTAA 1261 CAACAGCTTT AAACCAAAAC CGCTGCGTGG CTCTGCCTGA AGAGCTTTGA GACCCTGGAA 1321 TCAGCCTTGT GTCTGTCTTG AGGTATCTGA TTTTTTAGGG ATAACAGGGT AATGATCACA 1381 TCCGCCGTCT GTAGCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA 1441 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG 1501 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC 1561 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 1621 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 1681 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 1741 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 1801 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 1861 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 1921 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 1981 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 2041 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 2101 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG 2161 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 2221 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 2281 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 2341 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 2401 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 2461 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA 2521 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 2581 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 2641 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 2701 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2761 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2821 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2881 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2941 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 3001 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 3061 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 3121 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 3181 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 3241 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 3301 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC 3361 CGGAGGCTTT TGACTTGGTT CAGGTGGAGT GGGAG //