LOCUS Exported 3275 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3275 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1312..1359 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3207..3223 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3224..3255 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1180 /note="" /label="NotI (GC-rich recognition site)" primer_bind complement(1364..1380) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1447..2035) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2226..3086) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3087..3191) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1215..1274 /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." /label="FreeGenes Barcode" ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GCGTTCCGAC ACCTCCGTAG AACCGGAAGG 61 CGCCAACTTT ATCGCCGAGT TCTTCGGCCA CCGTGTATAT CCAGAGGTCG TTTCCACCGA 121 AGCTGCACGT AACGATCAGG CGACCGGTAC TTGTCCGTTC CTGACCGCCG CAAAACTTGT 181 TGAAACCAGC TGCGTAAAAG CGGAAACCTC TCGTGGTGTA TGCGTAGTGA ATACCGCAGT 241 AGACAACGAA CGCTACGACT GGCTGGTCTG TCCAAACCGT GCGCTGGACC CGCTGTTTAT 301 GAGCGCCGCA TCCCGCAAAC TGTTCGGCTA CGGTCCGACG GAGCCGCTGC AATTCATCGC 361 CGCGCCGACA CTGGCAGACC AGGCTGTACG TGATGGTATT CGTGAGTGGC TGGATCGCGG 421 TGTTCACGTT GTTGCTTACT TCCAAGAGAA GCTGGGCGGT GAATTAAGCA TTTCGAAAAC 481 CGATTCTTCT CCGGAGTTCT CTTTTGATTG GACCCTGGCC GAAGTTGAAT CTATCTACCC 541 GGTCCCGAAA ATCAAACGTT ACGGTGTTCT GGAAATCCAG ACCATGGATT TCCATGGTAG 601 CTATAAACAC GCAGTAGGTG CTATCGACAT CGCGCTGGTA GAAGGTATCG ACTTCCACGG 661 TTGGCTGCCG ACTCCGGCAG GTCGTGCAGC GCTGTCGAAA AAGATGGAAG GTCCAAACCT 721 GAGCAACGTT TTCAAACGTA CTTTTTACCA GATGGCGTAC AAATTCGCCC TGTCTGGCCA 781 TCAACGTTGC GCGGGTACTG GTTTCGCTAT CCCGCAATCC GTGTGGAAAT CCTGGCTGCG 841 CCATCTGGCA AACCCGACCC TGATTGACAA CGGTGACGGT ACTTTCAGCC TGGGTGATAC 901 CCGTAACGAC TCTGAGAACG CGTGGATTTT CGTGTTCGAA CTGGACCCGG ATACCGACGC 961 TTCCCCTAGA CCGCTGGCGC CGCACCTGGA AATTCGTGTT AACGTAGACA CTCTGATTGA 1021 TCTGGCTCTG CGCGAAAGCC CGCGCGCAGC CCTGGGTCCG AGCGGTCCGG TAGCGACCTT 1081 CACTGATAAG GTTGAAGCAC GTATGCTCCG TTTCTGGCCG AAAACCCGTC GTCGTCGCAG 1141 CACCACGCCT GGCGGCCAGC GTGGCCTCTT CGATGCCTGA AGAGCTTTGA GACCGTGTGG 1201 TCAATCCCAT GTCTACGAGG TTTTCGCATA ATGAGTAGGG ATAACAGGGT AATAAGCGCA 1261 AACGATTGCG GGTACGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA 1321 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG 1381 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC 1441 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 1501 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 1561 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 1621 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 1681 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 1741 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 1801 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 1861 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 1921 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 1981 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG 2041 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 2101 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 2161 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 2221 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 2281 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 2341 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA 2401 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 2461 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 2521 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 2581 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2641 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2701 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2761 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2821 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 2881 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 2941 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 3001 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 3061 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 3121 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 3181 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC 3241 CGGAGGCTTT TGACTTGGTT CAGGTGGAGT GGGAG //