LOCUS       Exported     3185 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3185
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1222..1269
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3117..3133
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3134..3165
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1090
                     /label="M.NotI"
                     /note=""
     primer_bind     complement(1274..1290)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1357..1945)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2136..2996)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2997..3101)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1125..1184
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GAGTGGTCCG CGTCTGCATG TAGGTATTTC
       61 TGTTTGTGGT TCTCTGACCG GGCTGTGGAT CACCCGTTCT CGTCGTACTC CGGTACGTGT
      121 AGTGATCCCG TATGATGCCT GCGTAACCTC CCTGAGCTCA TATGATTCTG CGATTGATGA
      181 TCCGACCGTG GAAGTTCGTC AGGGTGACGC CTACGACCTG GCAAGCGGTC TGGACCCGCA
      241 GTCGATCGAC CTGATTATCA CCAGCCCGCC GTATTGGGGT ATGCGTACCT ATGGCCACGA
      301 TCATTCTGAA GATGTACTGG ACGAATGGGT AGCTGAAGGT AACCACGCAA CCGACGTTCC
      361 ACCGTACGAA TGGTACCGTG AACATGGCGG CCTGTTGGGC ATGGAACCAA TTCCGGAATG
      421 GTTCATCTCT CACCTGGTGG AAATCTTCGA ACGTCTGCGT CCGGCCCTGA AGCTGGGCGG
      481 CAGCGTGTGG GTAAACCTGG GCGACACTTA TTTCGCGCGT TGGTCGTCCA TTCGTTCCGA
      541 CGGCCGTCAG GGCCTCGGTG ATAATCCACG CACACGTCGC AAAACCCCGA TGGGTGGTTA
      601 TCGCCAGGAG AAACAACTGA TGTTAATCCC GAGCCGTTTC GCCATCGCTA TGCAGGATAA
      661 ACGCTGGATT CTGCGCAACG ACCTGATCTG GCACAAACCG AACGTAGCGC CGCGCCCGGA
      721 AAAAGACCGT CTGCGTCTGG CCCATGAACA CTTCTTCCAC TTCGTGCTCC GCCCGAAGGA
      781 AGGTCGCGCT AAATACTACT ACGATACCAG TGCCGTTGAA GAAGGTACCC GTGACGTGGT
      841 TACTGTTAAC GTACGTTCCG GCAGCGACGG CCACTCTGCA ACTTTCCCGC CGGATCTGAT
      901 CAGACCGCGC ATCGAATCTT CTTCTCCGGT TGGTGGTCTG GTGCTGGACC CGTTCGCCGG
      961 TACCGGTCGT GCTCTGGGCG TCGCCGCGGA ATTAGGCCGT AGCGCAATCG GCTTCGAGCT
     1021 GTCGGAAGAA TTTACCCAGG CAGCAACCCG CAACGCTGAA GCTTCTGCAA ACGCACTGCG
     1081 CCTGCTGTGA AGAGCTTTGA GACCGAGGTC AATCATGCCT CTCTCCGCTG GCGGTACTCA
     1141 ATGCGTAGGG ATAACAGGGT AATATTTTGG GTACCCGTTA ATCCCGCTGA GGTGTCAATC
     1201 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1261 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1321 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1381 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1441 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1501 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1561 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1621 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1681 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1741 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1801 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1861 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1921 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     1981 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2041 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2101 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2161 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2221 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2281 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2341 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2401 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2461 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2521 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2581 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2641 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2701 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2761 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2821 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2881 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2941 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3001 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3061 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3121 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT
     3181 GGGAG
//