LOCUS       Exported     3389 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3389
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1426..1473
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3321..3337
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3338..3369
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1294
                     /label="M.SfiI"
                     /note=""
     primer_bind     complement(1478..1494)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1561..2149)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2340..3200)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3201..3305)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1329..1388
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GCGTAAGCCG AGCAGCGGCC AGCCAGCGGG
       61 CCTGGACGCC TGGGACGAAA CCCGTAGCCT GGCGACCCAT GCTGGCCCAG ATTTCGCACT
      121 GTACGTGGGC GACTCCCTGG ATTGCCTGGC TAAACTGCCA GACGAATCGA TCAACACAGT
      181 CGTTACCAGC CCGCCGTACT GGGCGGTTCG TGACTACGAA CATGATGAAC AGCTGGGCCT
      241 GGAGGACGAA GTTGATGACT ACGTGGAACG TCTGGTAAAA ATCTTTCGTG AGGTCTATCG
      301 CGTTCTGGCG ACTGACGGTT CTGCATGGCT GAACATCGGC GATTCTTACT TCAACAAGCA
      361 GATCACCGTT GGCGGCAAAC CGCCGCGTAC AGGTTGGAAA CGTAACAAAC AGCTCTCTCT
      421 GGTGCCTTTT CGTGTTGCCC TGGCCCTCCA GGACGACGGC TGGTGGATTC GCAATGTAGC
      481 AGTGTGGCAC AAACCAAACG CGATGCCGGC CAGCGTTCGT GATCGCCTGA CCGTTACCTG
      541 GGAACCGGTT TTTCTGCTGA CTAAATCCGA GCGTTACTAC TTCAATCTGG ATGAAATCCG
      601 CGTTCCACAT CAAACTAGCG ACGCAATTGA ACGTCGTCGC GCTGAAAGCG GCACCGTTAC
      661 TGGTAAAGCA CAGGGCAAAA AAGAGCTGCG TAAATGGCTG AACAGCCCGC GTCACCGTGC
      721 AACCATTGAA GGTATCAAAG AAGTTGAACG TCGTCCGAAC GCGCCGGCAG CAGTGGAACT
      781 CGCCAGCTAC CTGCGCACCG CCCTGAAAGA AAAGAAGCGC AGCATTGCAT GGGTAGCAGA
      841 ACAGCTGGAC CTGCCGTTCG AACGCACCCG CCATTACTTC CGCACCGATG AAATCGGCAG
      901 CCGTCTGCCG CCGCCGGAAG TGTGGGAACA GCTGAAAGAC CTGCTGGAAC TGGACGCTAC
      961 CTACGATGAG GCCATGACTG TGGAGGTGGG TGACAACGTT TTCCGTAACC ACCCAAACGG
     1021 CAAAAACCCG GGTGACCTGC TGAGCATCCC GACTGCACCG TCTGGTGCGA ACCACTTCGC
     1081 TGTTATGCCG CGTAAACTGG CGCACTTTGC CCTGAAAGCG ACCCTGCCGA TGAACGGCTC
     1141 CTGCCTGGAC CCGTTCATGG GCTCTGGTAC CACCGGCCGT GTTGTGCGTG AGCTGGGCGG
     1201 TCGTTTCGTG GGTGTCGACG TGAACGAGCA CTACATGACC GACTACCTGG TGGAAAGCGG
     1261 TGTGATCTCC CCGGAAACCG AAACCCTGTG GTGAAGAGCT TTGAGACCGC TTGACGGAGC
     1321 ATCTATCTCA GACGACAAGT CACCGCTGCT AGGGATAACA GGGTAATCAG CACTTTGCAT
     1381 GGTTTGTCCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1441 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1501 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     1561 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1621 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1681 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1741 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1801 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1861 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1921 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1981 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     2041 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2101 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     2161 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2221 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     2281 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2341 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2401 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     2461 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     2521 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     2581 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     2641 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     2701 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     2761 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     2821 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2881 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2941 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     3001 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     3061 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3121 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3181 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3241 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3301 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3361 CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//