LOCUS       Exported     3392 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3392
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1429..1476
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3324..3340
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3341..3372
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1297
                     /label="M.NcoI"
                     /note=""
     primer_bind     complement(1481..1497)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1564..2152)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2343..3203)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3204..3308)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1332..1391
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GTCCCAGCCA CTGCTGCCGC TGGACCTGCC
       61 GATGACCGGC CGTGGGACTA ACACTACTAC CTTTGTTGAC AACATGCGTC TGCCGGTTCA
      121 CCGTTGGTTT CGTTACTCCG CCGGTTTTTC TGCGCAATGG GCACAGAGCG TAATTGCGGA
      181 ATCCGGGGCG CAACGCGTTC TGGACCCGTA CTCCGGCTCT GGTACCACCG TGATCGCAGC
      241 TGAGGAATCT GGCGCGGCCG GCCTGGGCGT AGATGTTCAC CCGTTTGTTA CCCGCGTGGC
      301 GAAAGCCAAA CTGGCGTGGC GTGCAGAACC GGACGTGTTT CTGAAGCGCG CTAAAGCCGT
      361 GCGTATCGAG GCAGAGCGTC TGCAACCGGT GGAAGAACCA CCGGCACCGC TTATCGCAAA
      421 ATGTTTTCCA GATCGCCAGG CTCTGGTCGA CCTGCTGCGT ATCCGTGATG CGGTGGAACG
      481 TCTGCGTACC GGTGACCAGT ATGACGACCT GCTGTGGCTG GCGCTGGTTA GCATCATTCG
      541 TGCTTGCAGT CCAGCAGGCA CCGCTCAGTG GCAGTACGTT CTGCCGAACA AAACCAAATC
      601 ACGTGTTGCG GAGCCGCTCT CTGCGTTCGA CATTCGCACC GACCTGTTCG CACAGGATAT
      661 GTGGGCGATG CAGGAACACC ACGGTGCCCC GGAAGCACGT ATCATCGAAG CAGACGCTCG
      721 TACTCTGGAC GGCGTTGAGG ACGGCTGGGC GGACCTCATC CTGACCTCTC CGCCGTATGC
      781 GAACAATTTC GATTACGCGG ATGCTACCCG TCTGGAACAG TCCTTCCTGG GTGAGATTTC
      841 CGGCTGGGGC GACCTGAAAC CGCTGCGTAA GAAATTGATG AAATCTGCAA CTCAGCACAT
      901 GGGCGGTTGG GACGCCGCGG AAGCGCTGGA ATCTCCGCTG CTGGAACCGA TCCGCGCGGA
      961 ACTGCTGGGT GTGTTCGAAG AACTGAGCCA GGTAAAACGC ACTAAATCCG GCAACAAAGC
     1021 ATACGATCTG ATGGTTGCCG GCTATTTTCT GGACAGCGTG CAGACCCTGT ACGCGCTGCG
     1081 TCGCGCCAGC GCGCCGGGTG TTCAAGTCTG TTACGTTGTC GGTGACTCGG CCCCGTACGG
     1141 TGTTCACGTT CCTGTTGAGC GCTGGCTGGG TGAGCTGGCC CTGGCCGCGG GCTTCAAGTC
     1201 TTGGCGCTTC GAAAAAGTTC GTGATCGTAA CACCAAATGG AAGAATCGTA AACACGACCA
     1261 CCCGCTGCAC GAAGGTCGCT TGTGGATTGA GGGCTGAAGA GCTTTGAGAC CGTCCGTCGG
     1321 AGTAACAATC TGTCCCTGTA ACGAAAAGCG GGTAGGGATA ACAGGGTAAT TTCGCTCCTG
     1381 AACGGCTCCC TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1441 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1501 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1561 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1621 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1681 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1741 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1801 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1861 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1921 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1981 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     2041 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2101 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     2161 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2221 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2281 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2341 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2401 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2461 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2521 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2581 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2641 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2701 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2761 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2821 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2881 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2941 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     3001 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     3061 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     3121 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     3181 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     3241 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     3301 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3361 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AG
//