LOCUS Exported 3392 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3392 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1429..1476 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3324..3340 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3341..3372 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1297 /label="M.NcoI" /note="" primer_bind complement(1481..1497) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1564..2152) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2343..3203) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3204..3308) /note="ampicillin resistance gene promoter" /label="bla promoter" misc_feature 1332..1391 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GTCCCAGCCA CTGCTGCCGC TGGACCTGCC 61 GATGACCGGC CGTGGGACTA ACACTACTAC CTTTGTTGAC AACATGCGTC TGCCGGTTCA 121 CCGTTGGTTT CGTTACTCCG CCGGTTTTTC TGCGCAATGG GCACAGAGCG TAATTGCGGA 181 ATCCGGGGCG CAACGCGTTC TGGACCCGTA CTCCGGCTCT GGTACCACCG TGATCGCAGC 241 TGAGGAATCT GGCGCGGCCG GCCTGGGCGT AGATGTTCAC CCGTTTGTTA CCCGCGTGGC 301 GAAAGCCAAA CTGGCGTGGC GTGCAGAACC GGACGTGTTT CTGAAGCGCG CTAAAGCCGT 361 GCGTATCGAG GCAGAGCGTC TGCAACCGGT GGAAGAACCA CCGGCACCGC TTATCGCAAA 421 ATGTTTTCCA GATCGCCAGG CTCTGGTCGA CCTGCTGCGT ATCCGTGATG CGGTGGAACG 481 TCTGCGTACC GGTGACCAGT ATGACGACCT GCTGTGGCTG GCGCTGGTTA GCATCATTCG 541 TGCTTGCAGT CCAGCAGGCA CCGCTCAGTG GCAGTACGTT CTGCCGAACA AAACCAAATC 601 ACGTGTTGCG GAGCCGCTCT CTGCGTTCGA CATTCGCACC GACCTGTTCG CACAGGATAT 661 GTGGGCGATG CAGGAACACC ACGGTGCCCC GGAAGCACGT ATCATCGAAG CAGACGCTCG 721 TACTCTGGAC GGCGTTGAGG ACGGCTGGGC GGACCTCATC CTGACCTCTC CGCCGTATGC 781 GAACAATTTC GATTACGCGG ATGCTACCCG TCTGGAACAG TCCTTCCTGG GTGAGATTTC 841 CGGCTGGGGC GACCTGAAAC CGCTGCGTAA GAAATTGATG AAATCTGCAA CTCAGCACAT 901 GGGCGGTTGG GACGCCGCGG AAGCGCTGGA ATCTCCGCTG CTGGAACCGA TCCGCGCGGA 961 ACTGCTGGGT GTGTTCGAAG AACTGAGCCA GGTAAAACGC ACTAAATCCG GCAACAAAGC 1021 ATACGATCTG ATGGTTGCCG GCTATTTTCT GGACAGCGTG CAGACCCTGT ACGCGCTGCG 1081 TCGCGCCAGC GCGCCGGGTG TTCAAGTCTG TTACGTTGTC GGTGACTCGG CCCCGTACGG 1141 TGTTCACGTT CCTGTTGAGC GCTGGCTGGG TGAGCTGGCC CTGGCCGCGG GCTTCAAGTC 1201 TTGGCGCTTC GAAAAAGTTC GTGATCGTAA CACCAAATGG AAGAATCGTA AACACGACCA 1261 CCCGCTGCAC GAAGGTCGCT TGTGGATTGA GGGCTGAAGA GCTTTGAGAC CGTCCGTCGG 1321 AGTAACAATC TGTCCCTGTA ACGAAAAGCG GGTAGGGATA ACAGGGTAAT TTCGCTCCTG 1381 AACGGCTCCC TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC 1441 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA 1501 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT 1561 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1621 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 1681 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 1741 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1801 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 1861 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 1921 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1981 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 2041 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 2101 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT 2161 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 2221 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 2281 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 2341 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC 2401 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC 2461 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA 2521 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC 2581 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 2641 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA 2701 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA 2761 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA 2821 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT 2881 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 2941 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG 3001 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA 3061 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC 3121 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG 3181 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 3241 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 3301 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG 3361 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AG //