LOCUS       Exported     3680 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3680
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1717..1764
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3612..3628
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3629..3660
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1585
                     /label="M.HindIII"
                     /note=""
     primer_bind     complement(1769..1785)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1852..2440)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2631..3491)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3492..3596)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1620..1679
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GGACGAGTCT AAAAAAATCT CCCTGGGCCA
       61 GTTCTTTACC CCGACCCACA TTGTGAAGTA CATGATCGGC CTGATGACTA AAAACAAAAA
      121 CGCGTCTATC CTCGAACCGA GCTCAGGTAA CGGTGTGTTC CTGGATTCCC TTATTCAGCT
      181 TGGCTATACG AACCTGACAA GTTACGAAAT TGACGGTGAC ATTATCTCGC ATCCGTTTGT
      241 CATCAACTCT TCCTTCATCA CGTCTTACGA TAAACCGCAG TATGACAGCA TCATCGGCAA
      301 CCCGCCATAC GTCCGTTGGA AAAACCTGTC CGAGCTACAG AAAAAAGAGC TGAAAGACAA
      361 CAGCATTTGG AAAATGTACT GCAATTCTCT GTGCGATTAC TTTTACATTT TCATCATCAA
      421 ATCTATCCTG CAACTGAAAG TAGGTGGTGA ACTGATTTTC ATCTGTCCGG ATTACTTCTT
      481 CTCCACTAAA AACGCCGAAG GTCTGCGCAA ATTCCTGATC AACAACGGTA GCTTCGAGAA
      541 AATCATCCTG TTCAACGAAT CTAAAGTTTT CCACGGCGTT TCCTCTTCCG TAGTAATTTT
      601 CAAATACATC AAAGGTAAAA ACATCGACAA CATTAACATT ATCAACATCG ACTCTAAGTC
      661 TCCGATCAAG AGCGAAGATA TCGAATCCCT GGGCGAATCT TATTACATTC CGCGTTTTTC
      721 TTCCTCCGAC GTGTGGGTAA CCAGTCCGAA CCACATCAAA GTGGCACTGG ACAAATTCGA
      781 AAGTTACTGC AAGACCATCA AAAAGGTGCA GCCTAAATCT CTGTTCGACG ATCTGGAGTT
      841 CAGCCGTATC GGTTCTGTTT GCGATATTGG CAACGGCATG GTGAGCGGCC TGGATAAAGC
      901 GTTCCAGATG AACGATATCA ACTACTCCGA ACTGGAACTG CTGAACAGCA TCTGCGTTGC
      961 GAAAGCAAAA CATCTGGACG CGTTCTGCTT CTCTGGTTAC ACCCGTTATA AATTCATCCT
     1021 TGATGACATC AACGAAGATA AACTGATCAC CTATTTCCCG AACTTCTTCT ACGAATTTAA
     1081 CAACTACAAG GACTATCTGC TGAAGCGCTA CTCTTACAAC AAATACCTGC CGTATTGGAA
     1141 ATGGGCGTTT CTGCGCAACT TCTCCCTGTT CAGCAAAAAT GAAAAGAAAA TCTTCGTTCC
     1201 GTGTAAAGAA CGTATCAGCA AAAAATCTAA CTTTCGTTTC TCTCTGGTAG ACGAATTTAT
     1261 TTACCCGACC CAGGACGTTA CTGCTCTGTA CAAAAAAGAG AACGTTAAAG AATCCATTGA
     1321 ATATATCACG GCTTACCTGA ACTCCAAAGC GGTATTCCTG TGGATGAAAT ATAAAGGCGT
     1381 TGTGAAAGGT AACGTTGTCG AGTTCAGCGA AAAACCTCTG GCTAATATCC CGTTCCGCCG
     1441 CATCGATTGG CAGCTGAAAT CTGAAAAAAA AATCCACGAC GACATCACGA ATCTTGTACG
     1501 CAAATACCTG TCCAATAAGG AGTTCAGCAT CCTGCACGAA ATCAATCTGA ATCTCGAAAA
     1561 ACTGGGCATT AAAGTTGAAA TTTGAAGAGC TTTGAGACCC CGAGATTTAG ACGGATGCTA
     1621 TAACCACGAT ATAACGTGGC TAGGGATAAC AGGGTAATAA TTCGAAGTCT CTTACCACAC
     1681 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1741 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1801 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1861 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1921 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1981 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     2041 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     2101 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     2161 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     2221 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     2281 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     2341 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2401 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     2461 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     2521 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     2581 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     2641 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2701 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2761 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2821 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2881 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2941 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     3001 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     3061 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     3121 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     3181 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     3241 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     3301 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     3361 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     3421 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     3481 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     3541 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     3601 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     3661 TGGTTCAGGT GGAGTGGGAG
//