LOCUS       Exported     2888 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2888
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      925..972
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2820..2836
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2837..2868
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..793
                     /label="DpnI"
                     /note=""
     primer_bind     complement(977..993)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1060..1648)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1839..2699)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2700..2804)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    828..887
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GGAGCTTCAC TTTAACTTAG AATTGGTTGA
       61 AACCTATAAA TCTAATTCTC AAAAGGCTCG TATTTTGACC GAAGATTGGG TCTATCGTCA
      121 GTCATATTGC CCGAACTGTG GCAATAACCC CTTAAACCAC TTTGAAAACA ATCGCCCAGT
      181 CGCGGACTTC TACTGCAATC ATTGCAGTGA AGAGTTCGAA TTAAAATCGA AAAAAGGTAA
      241 TTTTAGCTCC ACGATCAATG ATGGAGCATA TGCCACCATG ATGAAACGGG TTCAGGCGGA
      301 CAACAATCCT AATTTTTTCT TTCTGACCTA CACTAAAAAT TTTGAAGTAA ATAACTTCCT
      361 GGTGCTGCCG AAACAGTTTG TGACACCGAA ATCGATTATC CAGCGCAAAC CGCTCGCGCC
      421 GACAGCCCGT CGTGCAGGTT GGATTGGCTG TAACATTGAT CTGAGCCAAG TTCCTAGTAA
      481 AGGCCGTATC TTTTTGGTCC AAGACGGGCA GGTACGCGAT CCAGAGAAAG TGACTAAAGA
      541 ATTTAAACAG GGCCTGTTCC TGCGCAAGTC CTCGCTGTCA AGCCGTGGTT GGACGATCGA
      601 AATACTGAAC TGTATCGACA AGATTGAGGG TAGCGAATTT ACGCTCGAAG ATATGTATCG
      661 ATTCGAAAGT GATCTGAAAA ACATCTTCGT GAAAAACAAC CATATTAAGG AAAAAATACG
      721 CCAGCAGCTT CAAATTCTGC GCGATAAAGA GATTATCGAG TTCAAGGGCC GGGGGAAATA
      781 CAGAAAGCTG TGAAGAGCTT TGAGACCGCC CGGCAATTCT TATAGCTCGA TCTGTGTAAC
      841 GGACAGCATA GGGATAACAG GGTAATTCAG AATGCCTTCG TTAGATTCGC TGAGGTGTCA
      901 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
      961 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1021 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1081 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1141 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1201 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1261 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1321 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1381 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1441 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1501 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1561 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1621 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     1681 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     1741 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     1801 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     1861 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     1921 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     1981 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     2041 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     2101 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     2161 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2221 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     2281 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     2341 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     2401 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     2461 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     2521 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     2581 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     2641 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     2701 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     2761 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     2821 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG
     2881 AGTGGGAG
//