LOCUS Exported 3893 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3893 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1930..1977 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3825..3841 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3842..3873 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1798 /label="Bst HF" /note="" primer_bind complement(1982..1998) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(2065..2653) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2844..3704) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(3705..3809) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1833..1892 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GGAGGCCAAA GGCGAAAAGC CGCTGGAGGA 61 AATGGAGTTT GCGATCGTGG ATGTTATTAC CGAAGAGATG CTGGCAGATA AGGCAGCGTT 121 GGTGGTGGAA GTTATGGAGG AAAACTACCA CGATGCACCG ATTGTAGGTA TTGCGCTGGT 181 TAACGAACAC GGTCGTTTCT TTATGCGTCC GGAAACCGCT CTGGCGGACA GCCAGTTCCT 241 GGCGTGGCTG GCAGACGAAA CCAAAAAGAA ATCCATGTTC GACGCGAAAC GCGCTGTGGT 301 CGCTCTGAAG TGGAAAGGCA TCGAACTGCG CGGCGTTGCA TTTGACCTGT TACTGGCAGC 361 GTATCTTCTG AACCCGGCTC AGGACGCTGG TGATATCGCG GCTGTTGCTA AAATGAAACA 421 ATATGAGGCC GTGCGTTCTG ATGAAGCTGT GTACGGTAAA GGTGTTAAGC GTTCGCTGCC 481 GGATGAGCAG ACGCTGGCCG AACACCTGGT TCGCAAAGCC GCTGCGATCT GGGCCCTGGA 541 ACAGCCGTTC ATGGACGACC TGCGTAACAA CGAACAGGAC CAGCTGCTGA CCAAACTGGA 601 ACACGCTCTG GCTGCTATCC TGGCCGAAAT GGAGTTCACT GGTGTAAACG TTGACACTAA 661 ACGCCTGGAA CAGATGGGTT CGGAACTGGC TGAACAGCTT CGCGCGATTG AACAGCGTAT 721 CTATGAACTG GCCGGCCAGG AGTTCAACAT CAATTCCCCG AAACAGCTGG GCGTGATCCT 781 GTTCGAGAAG TTACAGCTGC CAGTTCTGAA AAAAACGAAA ACCGGTTACA GCACCAGCGC 841 CGACGTGCTG GAAAAATTAG CGCCGCACCA CGAGATCGTA GAGAACATTC TGCATTACCG 901 TCAGCTGGGT AAACTGCAAT CTACCTATAT CGAAGGTCTG CTGAAAGTTG TCCGTCCGGA 961 TACCGGCAAA GTGCACACGA TGTTCAATCA GGCGCTGACC CAGACGGGGC GCTTATCTAG 1021 CGCGGAGCCG AACCTGCAAA ACATCCCAAT CCGCCTGGAA GAAGGTCGTA AAATCCGTCA 1081 AGCTTTTGTG CCGTCCGAGC CGGATTGGTT AATTTTCGCG GCTGATTATA GCCAAATCGA 1141 ACTGCGCGTA CTGGCGCACA TTGCTGACGA TGATAACCTG ATTGAAGCTT TCCAGCGTGA 1201 CCTGGACATC CACACCAAAA CCGCAATGGA TATCTTTCAG TTAAGCGAAG AAGAAGTTAC 1261 CGCCAACATG CGTCGTCAAG CCAAAGCCGT AAACTTCGGT ATTGTTTACG GCATCTCCGA 1321 CTACGGCCTG GCTCAGAACC TGAACATCAC CCGTAAAGAA GCGGCTGAGT TCATCGAACG 1381 TTATTTCGCT TCCTTCCCGG GTGTAAAGCA GTATATGGAA AACATTGTTC AGGAAGCGAA 1441 ACAGAAAGGT TATGTTACCA CCCTCCTGCA TCGTCGCCGT TACCTGCCAG ACATCACCAG 1501 CCGCAACTTC AACGTGCGCT CCTTCGCCGA ACGCACCGCG ATGAACACGC CGATCCAAGG 1561 TTCGGCGGCG GATATCATTA AAAAAGCAAT GATTGACCTC GCGGCGCGTC TGAAAGAAGA 1621 ACAGCTGCAA GCTCGTCTGC TGTTACAGGT TCACGACGAA CTGATTCTGG AAGCACCGAA 1681 AGAAGAGATC GAACGCCTGT GCGAACTGGT CCCGGAAGTG ATGGAACAGG CAGTGACCCT 1741 GCGTGTTCCG CTCAAAGTGG ACTACCACTA TGGCCCGACT TGGTACGACG CCAAGTGAAG 1801 AGCTTTGAGA CCGTTACACC GGGATCATAG CTCATCAAAT CTACCGTATT AGCTAGGGAT 1861 AACAGGGTAA TACTCATGGA GGGTCCAGGC TGCGCTGAGG TGTCAATCGT CGGAGCCGCT 1921 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT 1981 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG 2041 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 2101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 2161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 2221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 2281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 2341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 2401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 2461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 2521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 2581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 2641 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG 2701 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 2761 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 2821 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 2881 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 2941 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG 3001 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 3061 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 3121 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 3181 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 3241 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 3301 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 3361 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 3421 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 3481 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 3541 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 3601 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 3661 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 3721 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 3781 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG 3841 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAG //