LOCUS       Exported     4154 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4154
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2191..2238
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4086..4102
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4103..4134
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..2059
                     /label="Tth DNA Ligase K294R"
                     /note=""
     primer_bind     complement(2243..2259)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2326..2914)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3105..3965)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3966..4070)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2094..2153
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GACCCTGGAA GAAGCACGTA AACGTGTGAA
       61 CGAACTGCGT GATCTGATCC GTTATCACAA CTACCGCTAT TATGTGCTGG CGGACCCGGA
      121 AATTTCTGAT GCTGAATACG ATCGCCTGCT CCGCGAACTT AAAGAGCTGG AAGAACGCTT
      181 CCCGGAACTG AAAAGCCCGG ATTCCCCGAC TCTGCAAGTT GGCGCTCGCC CGCTGGAAGC
      241 GACCTTCCGT CCGGTGCGTC ACCCGACCCG TATGTATAGC CTGGATAACG CTTTCAACCT
      301 CGACGAACTA AAAGCGTTCG AGGAACGCAT TGAACGTGCG CTGGGTCGTA AAGGCCCGTT
      361 CGCCTATACT GTTGAGCACA AGGTTGATGG TCTGTCTGTA AACCTGTACT ACGAAGAGGG
      421 CGTTTTGGTT TATGGCGCGA CCCGTGGCGA CGGTGAGGTG GGTGAAGAGG TGACTCAGAA
      481 TCTGCTCACT ATCCCGACCA TCCCGCGTCG TCTTAAGGGT GTCCCTGAGC GTCTGGAAGT
      541 GCGTGGTGAA GTTTACATGC CAATCGAAGC TTTCCTGCGT CTGAACGAGG AACTGGAGGA
      601 GCGTGGTGAG CGCATCTTCA AAAACCCGCG CAACGCAGCC GCCGGATCTC TGCGTCAGAA
      661 AGATCCGCGC ATTACCGCGA AACGCGGCCT GCGTGCTACC TTCTACGCTC TGGGTCTGGG
      721 CCTGGAAGAA GTAGAGCGTG AGGGTGTAGC AACCCAGTTT GCTCTGCTGC ACTGGCTCAA
      781 AGAAAAAGGC TTCCCGGTCG AACACGGCTA TGCACGTGCA GTTGGCGCAG AAGGCGTAGA
      841 AGCCGTGTAC CAGGACTGGC TGAAGAAACG TCGCGCTCTG CCGTTCGAAG CTGATGGCGT
      901 GGTTGTCAAA CTGGACGAAC TTGCTCTGTG GCGTGAACTG GGGTACACCG CGCGTGCCCC
      961 GCGTTTTGCA ATTGCATACA AATTCCCGGC TGAAGAAAAA GAAACCCGTC TGCTGGACGT
     1021 CGTTTTCCAG GTAGGCCGTA CTGGTCGTGT CACGCCGGTA GGCATTCTTG AACCGGTATT
     1081 CCTGGAAGGC TCAGAGGTGA GCCGTGTCAC CCTTCACAAC GAATCTTACA TCGAAGAACT
     1141 GGACATTCGT ATCGGCGACT GGGTGCTGGT TCACAAAGCA GGTGGCGTTA TTCCGGAGGT
     1201 ACTTCGCGTT CTGAAAGAAC GTCGTACAGG CGAAGAACGC CCGATTCGTT GGCCGGAAAC
     1261 CTGTCCAGAA TGCGGTCATC GCCTGCTGAA AGAAGGCAAA GTTCATCGTT GCCCGAACCC
     1321 GCTCTGCCCG GCTAAACGTT TCGAAGCAAT TCGCCACTTC GCTTCTCGCA AAGCGATGGA
     1381 TATCCAGGGG TTAGGAGAAA AACTGATCGA GCGTCTGCTG GAAAAGGGCC TGGTCAAAGA
     1441 CGTAGCTGAT CTTTACCGTC TGCGCAAAGA GGACCTGGTT GGCCTGGAAC GTATGGGCGA
     1501 AAAATCTGCA CAGAACCTGC TGCGTCAGAT CGAGGAAAGC AAAAAACGTG GCCTGGAGCG
     1561 CCTGCTGTAC GCGCTGGGGT TACCGGGCGT GGGTGAAGTG CTGGCTCGTA ACCTGGCGGC
     1621 TCGTTTCGGC AACATGGATC GCCTGCTGGA GGCGAGCCTG GAAGAACTCC TGGAAGTGGA
     1681 AGAAGTTGGC GAACTAACGG CGCGTGCCAT CCTGGAAACT CTGAAAGACC CGGCCTTCCG
     1741 CGATCTGGTC CGCCGCCTCA AGGAAGCGGG CGTAGAAATG GAAGCGAAAG AAAAAGGCGG
     1801 TGAAGCACTG AAAGGGCTGA CTTTCGTTAT CACCGGCGAA CTGTCCCGTC CTCGTGAAGA
     1861 GGTGAAAGCC CTGCTGCGTC GCCTGGGCGC AAAAGTGACT GACTCTGTTA GCCGTAAGAC
     1921 TAGCTATCTG GTTGTTGGTG AAAACCCGGG CTCTAAACTG GAAAAAGCAC GCGCTCTGGG
     1981 TGTACCAACT CTGACTGAAG AGGAGCTGTA CCGTCTGCTG GAGGCCCGCA CTGGTAAAAA
     2041 AGCTGAAGAA CTGGTGTGAA GAGCTTTGAG ACCCGCTGAT GGTAATTAGC CCTTACACCT
     2101 CAGCAGGACG AGTTTAGGGA TAACAGGGTA ATGCACACAC CCAGCCTCAT ATCCGCTGAG
     2161 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     2221 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     2281 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     2341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     2401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     2461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     2521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     2581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     2641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     2701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     2761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2941 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     3001 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     3061 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     3121 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     3181 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     3241 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     3301 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     3361 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     3421 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     3481 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     3541 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     3601 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     3661 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     3721 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     3781 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     3841 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3901 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3961 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     4021 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     4081 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC
     4141 AGGTGGAGTG GGAG
//