LOCUS       Exported     3029 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3029
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1066..1113
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2961..2977
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2978..3009
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..934
                     /label="T4 PNK"
                     /note=""
     primer_bind     complement(1118..1134)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1201..1789)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1980..2840)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2841..2945)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    969..1028
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GAAAAAGATC ATCCTGACTA TCGGTTGCCC
       61 GGGTTCCGGC AAATCTACCT GGGCACGTGA GTTCATCGCT AAAAACCCAG GTTTCTATAA
      121 CATCAACCGC GATGATTACC GTCAGTCTAT TATGGCTCAC GAAGAACGCG ATGAATACAA
      181 ATACACTAAA AAAAAAGAAG GTATCGTCAC CGGTATGCAG TTCGATACTG CCAAGTCTAT
      241 CCTGTACGGC GGTGATTCCG TTAAAGGCGT CATCATCTCC GACACGAACC TGAACCCAGA
      301 GCGCCGCCTG GCGTGGGAAA CGTTCGCTAA AGAGTATGGT TGGAAAGTTG AGCACAAAGT
      361 GTTTGATGTA CCGTGGACCG AACTGGTAAA ACGTAACTCT AAACGTGGTA CCAAAGCAGT
      421 TCCAATCGAT GTTCTGCGCT CCATGTACAA ATCCATGCGC GAATACCTGG GCCTGCCGGT
      481 TTACAATGGC ACCCCGGGCA AGCCGAAGGC CGTCATCTTC GACGTTGACG GCACCCTGGC
      541 AAAGATGAAC GGCCGTGGTC CGTACGATCT GGAAAAATGT GACACCGACG TAATTAACCC
      601 GATGGTCGTT GAACTGTCCA AGATGTATGC CCTGATGGGT TACCAGATCG TTGTTGTTTC
      661 TGGTCGTGAG TCTGGTACTA AGGAAGATCC GACTAAGTAT TACCGTATGA CGCGTAAATG
      721 GGTCGAAGAT ATCGCTGGTG TTCCACTGGT AATGCAGTGC CAACGCGAAC AGGGCGACAC
      781 CCGTAAAGAT GACGTTGTAA AAGAAGAAAT CTTTTGGAAA CACATTGCAC CGCACTTCGA
      841 CGTGAAATTG GCGATTGACG ACCGTACCCA GGTGGTAGAA ATGTGGCGTC GCATTGGCGT
      901 AGAGTGCTGG CAGGTTGCGT CTGGTGATTT TTGAAGAGCT TTGAGACCGC AGCGATTCGG
      961 ATACTACTCT AAAGCCAACT AATATCCGGT AGGGATAACA GGGTAATGCG AAGCAGTCAC
     1021 GCGGCTACCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1081 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1141 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     1201 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1261 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1321 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1381 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1441 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1501 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1561 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1621 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1681 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1741 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     1801 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     1861 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     1921 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     1981 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2041 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     2101 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     2161 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     2221 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     2281 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     2341 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     2401 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     2461 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2521 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2581 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     2641 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     2701 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     2761 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     2821 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     2881 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     2941 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3001 CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//