LOCUS       Exported     3179 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3179
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1216..1263
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3111..3127
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3128..3159
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1084
                     /label="T4-BGT"
                     /note=""
     primer_bind     complement(1268..1284)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1351..1939)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2130..2990)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2991..3095)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1119..1178
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GAAAATCGCC ATCATCAACA TGGGTAACAA
       61 CGTAATCAAC TTCAAAACGG TGCCTAGCTC CGAAACTATT TACCTGTTCA AAGTTATCTC
      121 CGAAATGGGC TTAAACGTCG ATATCATCTC TCTGAAGAAC GGTGTGTATA CTAAATCTTT
      181 TGATGAAGTT GATGTTAATG ATTACGATCG TCTTATCGTT GTTAACTCCT CCATTAACTT
      241 CTTTGGCGGC AAGCCGAACC TGGCTATCCT GTCTGCCCAA AAATTCATGG CAAAGTACAA
      301 GTCTAAAATC TATTATCTGT TCACCGACAT TCGTCTGCCG TTTAGTCAGA GTTGGCCGAA
      361 TGTCAAGAAC CGTCCGTGGG CGTATCTGTA CACTGAAGAG GAGCTGCTGA TCAAGAGCCC
      421 AATCAAAGTT ATCTCTCAGG GTATCAACCT GGATATCGCA AAAGCCGCTC ACAAGAAAGT
      481 TGACAACGTT ATCGAGTTCG AATACTTCCC GATCGAACAG TACAAAATCC ACATGAACGA
      541 CTTCCAGCTG TCCAAACCGA CCAAAAAAAC CTTGGATGTG ATTTATGGGG GCAGTTTCCG
      601 TTCTGGTCAG CGCGAGAGCA AAATGGTGGA GTTCCTGTTC GATACCGGCC TCAATATTGA
      661 GTTCTTTGGT AACGCACGTG AAAAACAATT TAAAAACCCG AAATACCCAT GGACCAAAGC
      721 TCCGGTTTTT ACCGGCAAAA TCCCGATGAA CATGGTGTCT GAGAAAAACT CTCAGGCTAT
      781 TGCTGCGCTG ATCATCGGTG ACAAAAACTA CAACGATAAT TTTATCACCC TGCGTGTCTG
      841 GGAAACTATG GCTTCTGACG CGGTTATGCT GATCGACGAA GAATTTGATA CCAAACACCG
      901 TATCATCAAC GACGCGCGTT TTTACGTCAA CAACCGTGCG GAACTGATCG ACCGTGTTAA
      961 CGAACTGAAA CATTCTGATG TTCTGCGTAA AGAGATGCTG TCCATCCAGC ACGATATTCT
     1021 CAACAAAACC CGTGCAAAAA AAGCTGAGTG GCAGGATGCA TTCAAAAAAG CTATCGATCT
     1081 GTGAAGAGCT TTGAGACCGT TGCCCTTGCT GAGATACTAG GTCCAGGTCT TAAGTGATTT
     1141 AGGGATAACA GGGTAATACT CCGGTCTAAT TTAGCCCACG CTGAGGTGTC AATCGTCGGA
     1201 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1261 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1321 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1381 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1441 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1501 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1561 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1621 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1681 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1741 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1801 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1861 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1921 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1981 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2041 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2101 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2161 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2221 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2281 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2341 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2401 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2461 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2521 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2581 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2641 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2701 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2761 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2821 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2881 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2941 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3001 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3061 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3121 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//