LOCUS Exported 3179 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3179 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1216..1263 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3111..3127 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3128..3159 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1084 /label="T4-BGT" /note="" primer_bind complement(1268..1284) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1351..1939) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2130..2990) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2991..3095) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1119..1178 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GAAAATCGCC ATCATCAACA TGGGTAACAA 61 CGTAATCAAC TTCAAAACGG TGCCTAGCTC CGAAACTATT TACCTGTTCA AAGTTATCTC 121 CGAAATGGGC TTAAACGTCG ATATCATCTC TCTGAAGAAC GGTGTGTATA CTAAATCTTT 181 TGATGAAGTT GATGTTAATG ATTACGATCG TCTTATCGTT GTTAACTCCT CCATTAACTT 241 CTTTGGCGGC AAGCCGAACC TGGCTATCCT GTCTGCCCAA AAATTCATGG CAAAGTACAA 301 GTCTAAAATC TATTATCTGT TCACCGACAT TCGTCTGCCG TTTAGTCAGA GTTGGCCGAA 361 TGTCAAGAAC CGTCCGTGGG CGTATCTGTA CACTGAAGAG GAGCTGCTGA TCAAGAGCCC 421 AATCAAAGTT ATCTCTCAGG GTATCAACCT GGATATCGCA AAAGCCGCTC ACAAGAAAGT 481 TGACAACGTT ATCGAGTTCG AATACTTCCC GATCGAACAG TACAAAATCC ACATGAACGA 541 CTTCCAGCTG TCCAAACCGA CCAAAAAAAC CTTGGATGTG ATTTATGGGG GCAGTTTCCG 601 TTCTGGTCAG CGCGAGAGCA AAATGGTGGA GTTCCTGTTC GATACCGGCC TCAATATTGA 661 GTTCTTTGGT AACGCACGTG AAAAACAATT TAAAAACCCG AAATACCCAT GGACCAAAGC 721 TCCGGTTTTT ACCGGCAAAA TCCCGATGAA CATGGTGTCT GAGAAAAACT CTCAGGCTAT 781 TGCTGCGCTG ATCATCGGTG ACAAAAACTA CAACGATAAT TTTATCACCC TGCGTGTCTG 841 GGAAACTATG GCTTCTGACG CGGTTATGCT GATCGACGAA GAATTTGATA CCAAACACCG 901 TATCATCAAC GACGCGCGTT TTTACGTCAA CAACCGTGCG GAACTGATCG ACCGTGTTAA 961 CGAACTGAAA CATTCTGATG TTCTGCGTAA AGAGATGCTG TCCATCCAGC ACGATATTCT 1021 CAACAAAACC CGTGCAAAAA AAGCTGAGTG GCAGGATGCA TTCAAAAAAG CTATCGATCT 1081 GTGAAGAGCT TTGAGACCGT TGCCCTTGCT GAGATACTAG GTCCAGGTCT TAAGTGATTT 1141 AGGGATAACA GGGTAATACT CCGGTCTAAT TTAGCCCACG CTGAGGTGTC AATCGTCGGA 1201 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT 1261 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT 1321 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA 1381 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 1441 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 1501 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 1561 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 1621 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 1681 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 1741 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 1801 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 1861 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 1921 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT 1981 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 2041 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 2101 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 2161 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 2221 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC 2281 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 2341 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 2401 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 2461 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 2521 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 2581 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 2641 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 2701 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 2761 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 2821 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 2881 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 2941 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 3001 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 3061 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC 3121 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAG //