LOCUS       Exported     3332 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3332
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1369..1416
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3264..3280
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3281..3312
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1237
                     /label="P45 (ArchaeMaxx/PfuTurbo)"
                     /note=""
     primer_bind     complement(1421..1437)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1504..2092)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2283..3143)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3144..3248)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1272..1331
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GCTGCACCAC GTGAAACTGA TCTACGCTAC
       61 TAAAAGCCGC AAACTGGTAG GTAAAAAAAT CGTGCTTGCG ATCCCTGGTT CCATCGCGGC
      121 GGTAGAGTGC GTTAAACTGG CGCGTGAACT GATTCGTCAC GGCGCAGAAG TCCACGCGGT
      181 AATGAGCGAA GCGGCAACCA AAATTATTCA CCCTTACGCG ATGGAGTTCG CCACCGGCAA
      241 CCCGGTGATC ACCGAAATTA CCGGTTTTAT CGAGCACGTA GAACTGGCCG GTGAACACGA
      301 AAACAAAGCT GATTTGATTC TGGTGTGTCC GGCTACCGCA AACACTATTT CTAAGATCGC
      361 CTGCGGTATC GATGACACTC CGGTTACCAC TGTCGTGACC ACCGCGTTCC CGCACATCCC
      421 GATCATGATC GCGCCGGCGA TGCACGAAAC CATGTATCGT CACCCGATCG TGCGTGAAAA
      481 CATTGAGCGC CTGAAAAAGC TGGGTGTAGA GTTCATTGGC CCTCGTATTG AGGAAGGCAA
      541 AGCAAAAGTG GCCAGCATTG ACGAAATCGT GTACCGTGTG ATCAAAAAAC TGCACAAAAA
      601 AACCTTAGAA GGTAAACGTG TACTGGTCAC CGCAGGCGCA ACCCGTGAAT ACATCGACCC
      661 TATCCGCTTC ATTACCAATG CAAGCAGCGG TAAAATGGGT GTCGCTCTGG CGGAAGAAGC
      721 TGATTTCCGT GGCGCTGAAG TTACCCTGAT TCGCACTAAA GGTTCCGTTA AAAGCTTTGT
      781 TGAAAACCAG ATTGAAGTTG AAACCGTAGA AGAAATGCTG AGCGCAATCG AAAACGAACT
      841 GCGCAGCAAA AAATATGACG TGGTAATCAT GGCGGCAGCT GTGTCGGACT TTCGTCCAAA
      901 AATCAAGGCA GAAGGCAAAA TCAAGAGCGA CCGCTCAATC ACAATTGAGC TGGTGCCGAA
      961 CCCGAAAATT ATCGACCGCA TCAAAGAGAT CCAGCCGAAC GTGTTCCTCG TTGGTTTTAA
     1021 GGCTGAAACC AGCAAAGAGA AACTTATCGA AGAGGGTAAG CGTCAGATCG AGCGCGCTAA
     1081 GGCCGATCTG GTGGTTGGTA ACACCCTGGA AGCTTTTGGC AGCGAAGAAA ACCAGGTAGT
     1141 TCTGATCGGC CGTGACTTTA CCAAAGAGCT GCCAAAAATG AAAAAACGTG AACTGGCAGA
     1201 GCGTATCTGG GACGAAATTG AAAAACTGCT CTCCTGAAGA GCTTTGAGAC CGTACGTCTG
     1261 AACTTGGGAC TGGTTAGCCG AAATATCAAG CGTAGGGATA ACAGGGTAAT AGTGTTGTAT
     1321 AGGTGCGACT GCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1381 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1441 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1501 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1561 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1621 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1681 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1741 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1801 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1861 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1921 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1981 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2041 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     2101 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2161 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2221 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2281 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2341 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2401 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2461 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2521 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2581 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2641 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2701 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2761 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2821 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2881 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     2941 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     3001 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     3061 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     3121 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     3181 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     3241 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3301 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AG
//