LOCUS       Exported     3653 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3653
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1690..1737
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3585..3601
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3602..3633
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1558
                     /label="Bovine DNTT"
                     /note=""
     primer_bind     complement(1742..1758)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1825..2413)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2604..3464)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3465..3569)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1593..1652
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GGACCCGCTG TGCACGGCAT CCAGCGGTCC
       61 GCGTAAGAAG CGCCCGCGCC AGGTGGGCGC TTCAATGGCA TCTCCACCTC ACGATATCAA
      121 ATTCCAGAAT CTGGTTTTGT TTATCCTGGA AAAGAAAATG GGCACGACCC GTCGTAACTT
      181 TCTGATGGAA CTCGCTCGCC GTAAAGGTTT CCGTGTAGAA AACGAACTGT CCGATAGCGT
      241 TACCCACATC GTAGCAGAAA ATAATAGCGG TTCTGAAGTT CTGGAATGGC TGCAAGTCCA
      301 GAACATCCGT GCGAGTTCCC AGCTGGAACT GCTGGACGTA TCTTGGTTGA TCGAATCTAT
      361 GGGGGCTGGC AAACCTGTTG AAATCACTGG TAAACACCAG CTGGTCGTTC GCACCGACTA
      421 TTCTGCAACT CCGAACCCGG GCTTTCAGAA AACCCCACCG CTGGCAGTTA AAAAAATCAG
      481 CCAGTACGCA TGCCAGCGTA AAACTACCCT GAACAACTAC AACCACATCT TTACCGACGC
      541 ATTCGAAATC CTGGCAGAAA ACTCTGAGTT CAAAGAGAAC GAAGTTAGCT ACGTGACTTT
      601 CATGCGTGCA GCGTCTGTTC TGAAATCCCT CCCGTTTACC ATCATCAGCA TGAAAGACAC
      661 CGAAGGCATC CCGTGTCTGG GCGATAAAGT TAAATGCATC ATCGAAGAAA TCATCGAGGA
      721 CGGCGAATCC TCCGAAGTTA AAGCTGTGCT CAACGATGAG CGCTATCAGT CCTTCAAACT
      781 GTTCACCTCC GTCTTTGGCG TGGGTCTGAA AACCTCCGAA AAATGGTTCC GTATGGGCTT
      841 CCGTTCTCTG TCTAAGATCA TGTCTGATAA AACTCTGAAG TTCACAAAAA TGCAGAAAGC
      901 GGGCTTTCTG TATTACGAGG ACCTGGTGTC GTGTGTGACC CGTGCTGAAG CTGAAGCGGT
      961 CGGTGTGCTG GTTAAAGAGG CCGTGTGGGC ATTCCTGCCG GATGCATTTG TCACCATGAC
     1021 GGGTGGTTTC CGTCGCGGCA AAAAAATCGG CCACGATGTC GATTTCCTGA TTACGAGTCC
     1081 GGGCTCCGCT GAGGACGAAG AACAACTGCT GCCGAAAGTA ATCAACCTGT GGGAAAAAAA
     1141 AGGGCTGCTG CTGTACTATG ATCTGGTTGA ATCCACATTC GAAAAATTTA AACTGCCAAG
     1201 CCGTCAAGTC GACACCCTGG ATCATTTCCA AAAATGCTTC CTGATTCTGA AACTGCATCA
     1261 CCAGCGTGTT GATTCTTCCA AATCGAACCA GCAGGAAGGT AAGACTTGGA AAGCTATTCG
     1321 CGTTGACCTG GTGATGTGCC CATACGAAAA CCGTGCGTTC GCCCTGCTGG GCTGGACCGG
     1381 TTCCCGTCAG TTCGAACGCG ACATCCGTCG TTATGCAACC CACGAACGTA AAATGATGCT
     1441 GGACAACCAC GCACTCTATG ACAAAACCAA ACGTGTGTTT CTGAAAGCCG AATCAGAAGA
     1501 GGAAATCTTT GCCCACCTGG GTCTGGATTA TATCGAACCG TGGGAACGTA ACGCCTGAAG
     1561 AGCTTTGAGA CCGTGTCACA TTGTGTCGGA CTGTAACAAT TGTATGGTGT CTTTAGGGAT
     1621 AACAGGGTAA TCTACAGCCG ACAGGGCTTA ACCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1681 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1741 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1801 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1861 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1921 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1981 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     2041 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     2101 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     2161 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2221 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2281 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2341 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2401 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2461 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2521 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2581 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2641 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2701 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2761 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2821 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2881 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2941 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3001 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3061 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3121 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3181 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3241 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3301 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3361 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3421 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3481 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3541 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3601 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAG
//