LOCUS       Exported     3068 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3068
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1105..1152
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3000..3016
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3017..3048
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..973
                     /note=""
                     /label="DNA Topoisomerase 1B Vaccinia Virus"
     primer_bind     complement(1157..1173)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1240..1828)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2019..2879)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2880..2984)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1008..1067
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GCGCGCCCTG TTCTACAAAG ATGGCAAACT
       61 GTTCACTGAC AACAATTTCC TGAACCCGGT TTCTGATGAC AACCCGGCAT ACGAAGTTCT
      121 GCAACACGTA AAAATTCCGA CCCACCTGAC CGATGTGGTG GTATACGAAC AAACTTGGGA
      181 AGAAGCGCTG ACGCGTCTGA TCTTCGTTGG CAGCGACTCC AAAGGTCGTC GTCAGTACTT
      241 CTATGGGAAA ATGCACGTAC AAAACCGCAA CGCCAAGCGT GATCGCATCT TTGTTCGTGT
      301 TTACAACGTG ATGAAACGTA TCAACTGCTT CATCAACAAA AACATCAAAA AGAGCTCTAC
      361 CGATTCCAAC TACCAGCTGG CTGTATTCAT GCTTATGGAA ACGATGTTCT TTATCCGTTT
      421 CGGCAAAATG AAATATCTGA AAGAAAACGA AACCGTTGGC CTCCTGACCC TGAAAAACAA
      481 ACACATCGAA ATCTCCCCGG ATGAAATTGT TATCAAGTTC GTTGGTAAGG ATAAAGTTAG
      541 CCATGAATTT GTTGTGCATA AATCCAACCG CCTGTACAAA CCGTTACTGA AACTCACGGA
      601 TGACAGCTCT CCTGAGGAGT TCCTGTTCAA CAAACTTTCA GAGCGTAAAG TGTACGAGTG
      661 CATCAAGCAG TTTGGCATCC GTATTAAAGA CCTGCGTACC TACGGTGTTA ATTATACTTT
      721 TTTGTACAAT TTCTGGACCA ATGTCAAATC TATCAGCCCG CTGCCTAGCC CGAAAAAGCT
      781 GATTGCCCTG ACCATTAAAC AGACTGCGGA GGTTGTGGGC CATACCCCGT CCATCAGCAA
      841 ACGCGCCTAC ATGGCCACCA CCATCCTGGA AATGGTAAAA GACAAAAACT TTCTGGACGT
      901 TGTGAGCAAA ACCACTTTCG ATGAGTTCCT GTCCATTGTT GTTGATCACG TTAAATCCTC
      961 AACTGACGGC TGAAGAGCTT TGAGACCGAG GATTCGGTCA GTTCACTCTC GAGTGCTGCC
     1021 GCAGAAGATA GGGATAACAG GGTAATTGGC GCCCATTATT ACGCTTTCGC TGAGGTGTCA
     1081 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1141 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1201 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1261 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1321 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1381 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1441 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1501 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1561 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1621 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1681 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1741 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1801 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     1861 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     1921 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     1981 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     2041 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2101 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     2161 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     2221 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     2281 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     2341 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2401 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     2461 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     2521 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     2581 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     2641 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     2701 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     2761 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     2821 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     2881 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     2941 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     3001 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG
     3061 AGTGGGAG
//