LOCUS       Exported     4273 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4273
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      2310..2357
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4205..4221
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4222..4253
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2178
                     /label="lambda red operon"
                     /note=""
     primer_bind     complement(2362..2378)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2445..3033)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3224..4084)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(4085..4189)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2213..2272
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GGATATTAAT ACTGAAACTG AGATCAAGCA
       61 AAAGCATTCA CTAACCCCCT TTCCTGTTTT CCTAATCAGC CCGGCATTTC GCGGGCGATA
      121 TTTTCACAGC TATTTCAGGA GTTCAGCCAT GAACGCTTAT TACATTCAGG ATCGTCTTGA
      181 GGCTCAGAGC TGGGCGCGTC ACTACCAGCA GCTCGCCCGT GAAGAGAAAG AGGCAGAACT
      241 GGCAGACGAC ATGGAAAAAG GCCTGCCCCA GCACCTGTTT GAATCGCTAT GCATCGATCA
      301 TTTGCAACGC CACGGGGCCA GCAAAAAATC CATTACCCGT GCGTTTGATG ACGATGTTGA
      361 GTTTCAGGAG CGCATGGCAG AACACATCCG GTACATGGTT GAAACCATTG CTCACCACCA
      421 GGTTGATATT GATTCAGAGG TATAAAACGA ATGAGTACTG CACTCGCAAC GCTGGCTGGG
      481 AAGCTGGCTG AACGTGTCGG CATGGATTCT GTCGACCCAC AGGAACTGAT CACCACTCTT
      541 CGCCAGACGG CATTTAAAGG TGATGCCAGC GATGCGCAGT TCATCGCATT ACTGATCGTT
      601 GCCAACCAGT ACGGCCTTAA TCCGTGGACG AAAGAAATTT ACGCCTTTCC TGATAAGCAG
      661 AATGGCATCG TTCCGGTGGT GGGCGTTGAT GGCTGGTCCC GCATCATCAA TGAAAACCAG
      721 CAGTTTGATG GCATGGACTT TGAGCAGGAC AATGAATCCT GTACATGCCG GATTTACCGC
      781 AAGGACCGTA ATCATCCGAT CTGCGTTACC GAATGGATGG ATGAATGCCG CCGCGAACCA
      841 TTCAAAACTC GTGAAGGCAG AGAAATCACG GGGCCGTGGC AGTCGCATCC CAAACGGATG
      901 TTACGTCATA AAGCCATGAT TCAGTGTGCC CGTCTGGCCT TCGGATTTGC TGGTATCTAT
      961 GACAAGGATG AAGCCGAGCG CATTGTCGAA AATACTGCAT ACACTGCGGA ACGTCAGCCG
     1021 GAACGCGACA TCACTCCGGT TAACGATGAA ACCATGCAGG AGATTAACAC TCTGCTGATC
     1081 GCCCTGGATA AAACATGGGA TGACGACTTA TTGCCGCTCT GTTCCCAGAT ATTTCGCCGC
     1141 GACATTCGTG CATCGTCAGA ACTGACACAG GCCGAAGCAG TAAAAGCTCT TGGATTCCTG
     1201 AAACAGAAAG CCGCAGAGCA GAAGGTGGCA GCATGACACC GGACATTATC CTGCAACGTA
     1261 CCGGGATCGA TGTGAGAGCT GTCGAACAGG GGGATGATGC GTGGCACAAA TTACGGCTCG
     1321 GCGTCATCAC CGCTTCAGAA GTTCACAACG TGATAGCAAA ACCCCGCTCC GGAAAGAAGT
     1381 GGCCTGACAT GAAAATGTCC TACTTCCACA CCCTGCTTGC TGAGGTTTGC ACCGGTGTGG
     1441 CTCCGGAAGT TAACGCTAAA GCACTGGCCT GGGGAAAACA GTACGAGAAC GACGCCAGAA
     1501 CCCTGTTTGA GTTCACTTCC GGCGTGAATG TTACTGAATC CCCGATCATC TATCGTGACG
     1561 AAAGTATGCG TACCGCCTGC TCTCCCGATG GTTTATGCAG TGACGGCAAC GGCCTTGAAC
     1621 TGAAATGCCC GTTTACCTCC CGGGATTTCA TGAAGTTCCG GCTCGGTGGT TTCGAGGCCA
     1681 TAAAGTCAGC TTACATGGCC CAGGTGCAGT ACAGCATGTG GGTGACGCGA AAAAATGCCT
     1741 GGTACTTTGC CAACTATGAC CCGCGTATGA AGCGTGAAGG CCTGCATTAT GTCGTGATTG
     1801 AGCGGGATGA AAAGTACATG GCGAGTTTTG ACGAGATCGT GCCGGAGTTC ATCGAAAAAA
     1861 TGGACGAGGC ACTGGCTGAA ATTGGTTTTG TATTTGGGGA GCAATGGCGA TGACGCATCC
     1921 TCACGATAAT ATCCGGGTAG GCGCAATCAC TTTCGTCTAC TCCGTTACAA AGCGAGGCTG
     1981 GGTATTTCCC GGCCTTTCTG TTATCCGAAA TCCACTGAAA GCACAGCGGC TGGCTGAGGA
     2041 GATAAATAAT AAACGAGGGG CTGTATGCAC AAAGCATCTT CTGTTGAGTT AAGAACGAGT
     2101 ATCGAGATGG CACATAGCCT TGCTCAAATT GGAATCAGGT TTGTGCCAAT ACCAGTAGAA
     2161 ACAGACGAAG AAGCCTGAAG AGCTTTGAGA CCGGTACGGT TTACAGACCA CTTTATGACG
     2221 CTTGCTGCCC GGGTAGGGAT AACAGGGTAA TCTCAAAGCA GTGCCGTTAA CTCGCTGAGG
     2281 TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG
     2341 TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC
     2401 ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC
     2461 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
     2521 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
     2581 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
     2641 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
     2701 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
     2761 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
     2821 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
     2881 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
     2941 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
     3001 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC
     3061 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
     3121 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
     3181 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
     3241 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
     3301 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
     3361 CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
     3421 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
     3481 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
     3541 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
     3601 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
     3661 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
     3721 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
     3781 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
     3841 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
     3901 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
     3961 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
     4021 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
     4081 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG
     4141 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA
     4201 GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA
     4261 GGTGGAGTGG GAG
//