LOCUS       Exported     2981 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2981
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1018..1065
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2913..2929
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2930..2961
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..886
                     /note=""
                     /label="Endonuclease IV E. coli"
     primer_bind     complement(1070..1086)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1153..1741)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1932..2792)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2793..2897)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    921..980
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GAAATACATC GGCGCGCACG TTTCCGCGGC
       61 GGGCGGTCTG GCGAACGCGG CGATTCGTGC CGCAGAAATT GACGCAACAG CATTCGCACT
      121 GTTCACCAAA AACCAGCGTC AATGGCGCGC GGCCCCGCTG ACCACCCAGA CTATCGATGA
      181 GTTCAAAGCG GCTTGTGAAA AGTACCATTA CACCTCCGCT CAGATCCTGC CGCACGACTC
      241 CTACCTTATT AATCTGGGCC ACCCGGTTAC AGAGGCTCTG GAAAAATCTC GTGATGCTTT
      301 CATCGATGAA ATGCAGCGTT GTGAGCAACT GGGTCTGTCT CTCCTGAACT TCCACCCGGG
      361 TTCGCACCTG ATGCAAATCT CTGAAGAAGA TTGTCTGGCA CGCATTGCGG AGTCTATCAA
      421 TATTGCGCTG GATAAGACCC AGGGCGTAAC CGCGGTTATT GAAAACACCG CGGGTCAGGG
      481 CTCGAATCTG GGTTTCAAGT TTGAACATTT GGCGGCTATC ATCGATGGCG TCGAGGACAA
      541 ATCTCGTGTC GGTGTGTGCA TCGACACTTG CCACGCGTTC GCTGCTGGTT ATGATCTGCG
      601 TACCCCGGCC GAATGTGAAA AAACCTTTGC GGACTTTGCA CGTACCGTTG GCTTTAAATA
      661 CCTGCGTGGC ATGCACCTGA ACGACGCTAA ATCTACCTTC GGTAGCCGTG TTGACCGCCA
      721 TCACAGTCTG GGTGAGGGCA ACATCGGACA CGACGCATTT CGTTGGATCA TGCAAGACGA
      781 TCGTTTCGAC GGTATCCCGC TGATCCTGGA AACCATTAAC CCAGATATCT GGGCGGAGGA
      841 AATCGCATGG CTGAAAGCTC AGCAGACCGA AAAAGCAGTT GCCTGAAGAG CTTTGAGACC
      901 GCGGGTTAGA TACTGCAACT GCTACTCATT CTGCTCCTTA TTAGGGATAA CAGGGTAATT
      961 GCCAATACGA GTTGTTTCCT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1021 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1081 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1141 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1201 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1261 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1321 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1381 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1441 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1501 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1561 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1621 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1681 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1741 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1801 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     1861 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     1921 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     1981 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2041 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2101 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2161 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2221 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2281 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2341 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2401 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2461 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2521 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     2581 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     2641 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     2701 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     2761 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     2821 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     2881 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     2941 TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA G
//