LOCUS       Exported     2930 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2930
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      967..1014
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2862..2878
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2879..2910
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..835
                     /note=""
                     /label="Exonuclease III E. coli"
     primer_bind     complement(1019..1035)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1102..1690)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1881..2741)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2742..2846)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    870..929
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GAAATTCGTT TCTTTCAACA TCAACGGGCT
       61 GCGCGCTCGT CCGCACCAGC TTGAAGCGAT CGTTGAGAAA CACCAGCCGG ACGTTATCGG
      121 CCTGCAAGAA ACCAAAGTTC ATGATGATAT GTTTCCGCTG GAGGAAGTAG CCAAACTTGG
      181 TTACAACGTT TTCTATCACG GTCAGAAAGG CCATTACGGT GTTGCTCTGC TGACTAAAGA
      241 AACCCCGATC GCGGTTCGTC GTGGCTTCCC GGGCGACGAT GAGGAAGCTC AGCGCCGCAT
      301 CATCATGGCA GAAATCCCGT CCCTGCTGGG CAACGTTACC GTGATTAACG GCTACTTCCC
      361 GCAAGGTGAA TCTCGTGACC ATCCGATCAA ATTCCCGGCT AAGGCACAGT TCTACCAGAA
      421 CCTGCAAAAC TATCTGGAAA CAGAACTCAA ACGCGATAAC CCAGTTCTGA TCATGGGCGA
      481 CATGAACATT TCGCCGACCG ATCTGGACAT CGGCATCGGT GAAGAAAACC GCAAACGCTG
      541 GCTGCGCACT GGTAAGTGCT CTTTCCTCCC GGAAGAACGC GAATGGATGG ATCGTCTGAT
      601 GAGCTGGGGT CTGGTGGACA CCTTCCGTCA TGCAAACCCG CAGACTGCGG ATCGTTTCTC
      661 CTGGTTCGAT TACCGTTCCA AAGGTTTCGA CGATAACCGT GGCCTGCGCA TTGACCTGCT
      721 GCTGGCTTCT CAGCCGCTGG CAGAATGCTG CGTCGAAACC GGCATCGACT ATGAAATTCG
      781 TTCTATGGAG AAACCTTCTG ATCACGCTCC GGTGTGGGCG ACATTCCGCC GTTGAAGAGC
      841 TTTGAGACCG TCTCATGCTA GCCACGTATC GCGGTCCTTG CCAGCTTCTA TAGGGATAAC
      901 AGGGTAATGT CATATCTTTG TTGACCCACC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
      961 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1021 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1081 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1141 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1201 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1261 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1321 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1381 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1441 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1501 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1561 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1621 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1681 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1741 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     1801 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     1861 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     1921 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     1981 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2041 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2101 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2161 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2221 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2281 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2341 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2401 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2461 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2521 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     2581 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2641 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2701 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     2761 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     2821 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     2881 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
//