LOCUS Exported 2798 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2798 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 835..882 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2730..2746 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2747..2778 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..703 /label="spisPink/spisCP Biobrick K1033925" /note="" primer_bind complement(887..903) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(970..1558) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1749..2609) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2610..2714) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 738..797 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GTCGCACTCA AAACAAGCAC TGGCAGATAC 61 GATGAAAATG ACGTGGCTGA TGGAAGGTTC GGTCAACGGT CACGCCTTTA CGATTGAAGG 121 CGAAGGTACC GGCAAACCGT ATGAAGGTAA ACAGAGTGGC ACCTTTCGTG TTACGAAAGG 181 CGGTCCGCTG CCGTTTGCGT TTGATATTGT CGCTCCGACC CTGAAATACG GTTTCAAATG 241 CTTCATGAAA TACCCGGCGG ATATTCCGGA CTACTTTAAA CTGGCCTTCC CGGAAGGCCT 301 GACGTACGAT CGTAAAATTG CGTTTGAGGA CGGCGGTTGT GCGACCGCCA CGGTCGAAAT 361 GAGCCTGAAG GGTAACACCC TGGTGCATAA AACGAACTTT CAGGGCGGTA ATTTCCCGAT 421 TGATGGTCCG GTGATGCAAA AACGCACCCT GGGCTGGGAA CCGACCTCTG AAAAAATGAC 481 GCCGTGCGAT GGTATTATCA AAGGCGACAC CATCATGTAC CTGATGGTTG AAGGCGGTAA 541 AACGCTGAAA TGTCGTTATG AAAACAATTA CCGCGCCAAC AAACCAGTGC TGATGCCGCC 601 GAGCCACTTT GTGGATCTGC GTCTGACCCG CACGAATCTG GATAAAGAAG GTCTGGCGTT 661 CAAACTGGAA GAATATGCTG TTGCCCGTGT GCTGGAAGTG TGAAGAGCTT TGAGACCCGC 721 ATGTAGGCAC CATCTATTAG CCACGAGTGC GTCCCTTTTA GGGATAACAG GGTAATTCAG 781 TGCTGGGTGC CAGATTGCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA 841 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC 901 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA 961 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 1021 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 1081 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 1141 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 1201 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 1261 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 1321 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 1381 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 1441 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 1501 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 1561 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 1621 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 1681 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 1741 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 1801 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 1861 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA 1921 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 1981 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 2041 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 2101 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 2161 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 2221 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 2281 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 2341 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 2401 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 2461 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 2521 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 2581 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 2641 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 2701 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC 2761 GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAG //