LOCUS Exported 2846 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2846 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 883..930 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2778..2794 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2795..2826 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..751 /note="" /label="fuGFP" primer_bind complement(935..951) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1018..1606) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1797..2657) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2658..2762) /note="ampicillin resistance gene promoter" /label="bla promoter" misc_feature 786..845 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GGTTTCTAGC GGTGAAGATA TCTTCTCAGG 61 CCTGGTCCCG ATTCTGATCG AGCTGGAGGG CGATGTGAAT GGCCACCGCT TCTCTGTTCG 121 TGGTGAAGGT TATGGCGACG CCTCTAACGG CAAACTGGAA ATTAAGTTCA TCTGCACCAC 181 CGGTCGTCTG CCGGTTCCGT GGCCGACGCT GGTGACTACT CTGTCTTACG GTGTCCAGTG 241 CTTCGCAAAA TACCCGGAAC ACATGCGTCA GAATGATTTT TTCAAGTCCG CCATGCCAGA 301 TGGCTATGTT CAGGAACGTA CCATCAGCTT CAAAGAGGAC GGTACGTACA AAACCCGTGC 361 GGAAGTTAAA TTTGAAGGTG AAGCGCTGGT GAACCGTATC GACTTAAAAG GTCTGGAGTT 421 CAAGGAAGAT GGCAACATTC TGGGCCACAA ACTGGAATAC AGCTTCAACA GCCACTATGT 481 TTATATTACC GCGGACAAAA ATCGCAACGG CCTGGAGGCG CAGTTCCGTA TCCGTCACAA 541 CGTCGACGAC GGTTCTGTCC AGCTTGCGGA CCATTACCAG CAGAACACCC CGATCGGTGA 601 GGGCCCAGTA CTGCTCCCGG AGCAACACTA CCTGACGACT AACTCTGTCC TGTCCAAAGA 661 CCCGCAGGAA CGCCGTGATC ACATGGTTCT GGTTGAATTT GTGACCGCTG CCGGCCTGTC 721 GCTGGGCATG GACGAACTGT ATAAATCCTG AAGAGCTTTG AGACCCCGAT AGTTCCAGCT 781 AGCATATAAA TTGTCCCAGT GCGTAGTAGG GATAACAGGG TAATTCCTCC AATTACGTAA 841 TCGCGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG 901 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG 961 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT 1021 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 1081 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 1141 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 1201 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 1261 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 1321 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 1381 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 1441 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 1501 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 1561 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT 1621 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 1681 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 1741 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 1801 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 1861 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 1921 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 1981 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 2041 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 2101 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 2161 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 2221 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 2281 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 2341 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 2401 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 2461 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 2521 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 2581 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 2641 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 2701 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 2761 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT 2821 TTGACTTGGT TCAGGTGGAG TGGGAG //