LOCUS Exported 2819 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2819 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 856..903 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2751..2767 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2768..2799 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..724 /label="aacuGFP1" /note="" primer_bind complement(908..924) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(991..1579) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1770..2630) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2631..2735) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 759..818 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GTCTCTGAGC AAACATGGTA TTACCCAGGA 61 AATGCCGACC AAGTACCACA TGAAAGGCTC CGTGAACGGC CACGAGTTCG AAATTGAAGG 121 TGTAGGCACC GGTCATCCGT ACGAAGGAAC CCATATGGCT GAGCTGGTGA TCATCAAACC 181 GGCCGGTAAA CCGCTTCCGT TCTCCTTCGA TATCCTGTCT ACTGTGATTC AGTACGGCAA 241 CCGTTGCTTC ACCAAGTACC CGGCGGATCT GCCGGACTAT TTCAAACAGG CGTATCCGGG 301 CGGCATGTCT TACGAACGTA GCTTCGTGTA CCAGGATGGC GGTATTGCAA CCGCGTCTTG 361 GAACGTGGGC CTGGAAGGCA ATTGTTTCAT CCACAAATCT ACTTATCTGG GTGTTAACTT 421 TCCGGCTGAC GGTCCAGTAA TGACCAAAAA AACCATCGGT TGGGACAAGG CTTTCGAAAA 481 AATGACTGGT TTCAACGAAG TTCTGCGTGG CGATGTTACC GAGTTCCTGA TGCTGGAGGG 541 CGGTGGCTAC CACTCTTGCC AGTTCCACTC CACCTATAAA CCGGAAAAGC CGGTGGAATT 601 GCCGCCGAAC CACGTAATTG AACATCATAT TGTTCGTACC GATCTTGGAA AAACCGCCAA 661 AGGTTTCATG GTGAAGCTGG TACAGCACGC GGCGGCACAC GTGAACCCGC TGAAAGTACA 721 GTGAAGAGCT TTGAGACCCG CTAAGATCCG TAGGTCTGAT CGGAGATCCC AAGATCGAGT 781 AGGGATAACA GGGTAATGTG CCTGGCGGAT AGCGGGACCG CTGAGGTGTC AATCGTCGGA 841 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT 901 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT 961 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA 1021 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 1081 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 1141 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 1201 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 1261 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 1321 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 1381 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 1441 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 1501 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 1561 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT 1621 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 1681 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 1741 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 1801 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 1861 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC 1921 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 1981 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 2041 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 2101 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 2161 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 2221 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 2281 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 2341 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 2401 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 2461 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 2521 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 2581 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 2641 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 2701 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC 2761 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAG //