LOCUS Exported 2804 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2804 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 841..888 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2736..2752 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2753..2784 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..709 /label="afraGFP" /note="" primer_bind complement(893..909) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(976..1564) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1755..2615) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2616..2720) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 744..803 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCTAAT GAGCGTTATT GTGAAAGAAA TGATGACCAA 61 ACTGCATATG GAAGGCACCG TTAACGGTCA CGCGTTCACA ATCGAAGGTA AGGGTAAAGG 121 CGATCCGTAC AACGGTGTTC AGTCTATGAA CCTGGACGTA AAAGGCGGTG CTCCTCTCCC 181 GTTCAGCTTC GACCTGCTGA CTCCGGCATT CATGTACGGC AACCGTGTGT TCACTAAATA 241 CCCGGAGGAC ATCCCTGACT TCTTTAAGCA GGTATTCCCG GAAGGCTATC ACTGGGAACG 301 TTCCATTACT TTTGAGGACC AGGCGGTGTG CACTGCAACC TCTCACATCC GCCTGGACCA 361 GAAAGAAATG TGCTTCATTT ATGACGTTCG TTTTCACGGC GTTAACTTCC CTGCAAACGG 421 CCCGATCATG CAGAAAAAAA TTCTGGGTTG GGAGCCGTCC ACCGAAAAAA TGTACGCTCG 481 TGACGGTGTA CTGAAAGGTG ATGTAAACAT GACCCTGCGC GTTGAGGGTG GCGGTCACTA 541 CCGTGCAGAT TTCCGTACCA CTTATAAAGC TAAAAAGCCG GTAAACCTGC CGGGCTACCA 601 CTTTATTGAT CACCGTATCG AAATCACGAA GCACTCTAAA GATTACACCA ACGTAGCGCT 661 GTACGAGGCA GCTGTTGCTC GTCATTCACC GCTGCCGAAA GTGGCCTGAA GAGCTTTGAG 721 ACCGTATGTC CCCTAAGCTC TGGTCCAGAC CTACCCAGGG TCCTTAGGGA TAACAGGGTA 781 ATAAAAAATA ATGCTCCGCA CGCCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA 841 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC 901 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA 961 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 1021 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 1081 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 1141 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 1201 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 1261 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 1321 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 1381 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 1441 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 1501 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 1561 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 1621 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 1681 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 1741 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 1801 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 1861 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT 1921 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 1981 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 2041 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 2101 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 2161 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 2221 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 2281 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 2341 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 2401 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 2461 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 2521 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 2581 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 2641 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 2701 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA 2761 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAG //