LOCUS Exported 2801 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2801 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 838..885 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2733..2749 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2750..2781 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..706 /label="sarcGFP" /note="" primer_bind complement(890..906) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(973..1561) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1752..2612) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2613..2717) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 741..800 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AACGATGATG CTCACTCTCG GGTCTCTAAT GTCCGTGATC AAACAGGAAA TGAAAATCAA 61 GCTGCACATG GACGGCAACG TTAACGGCCA CGCGTTCGTA ATCGAAGGCG ATGGCAAAGG 121 TAAACCGTAC GATGGCACCC AGACCCTGAA CCTGAGCGTC AAAGAAGGTG CGCCGCTGCC 181 GTTCTCCTAT GATATTCTGA CGAACGCGTT TCAGTACGGC AATCGCGCCT TCACTAAATA 241 CCCTGCGGAC ATCCCTGATT ACTTCAAACA GACCTTTCCT GAGGGCTATT CCTGGGAACG 301 TACCATGAGC TATGAAGATA ACGCCATCTG CAACGTGCGT TCTGAAATCT CCATGGAAGG 361 CGACTGCTTC ATCTACAAAA TCCGTTTCGA TGGCAAAAAC TTCCCGCCGA ACGGGCCGGT 421 AATGCAAAAA AAGACCCTGA AATGGGAACC GTCGACTGAA ATGATGTACG CTCGTGACGG 481 ATTCCTTATG GGCGATGTGA ATATGGCGCT GCTGCTGGAA GGTGGTGGTC ACCACCGTTG 541 TGATTTCAAA ACCTCCTACA AGGCAGCGAA AAAAAACGTA CAGCTGCCTG ACTACCATTA 601 CGTCGATCAT CGTATTGAAA TCCTGTCTCA TGACCGTGAC TACAACAAAG TGAAACTGTA 661 CGAAAACGCA GTGGCTCGCT ACAGCCTGCT GCCGAGCCAG GCCTGAAGAG CTTTGAGACC 721 GCTCGATGCC ATAGTTCCTT GGACGGTATG AGGTTCATGA CTAGGGATAA CAGGGTAATT 781 AGTATATGGT TTGGGCCGCG CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA 841 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT 901 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA 961 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 1021 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 1081 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 1141 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 1201 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 1261 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 1321 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 1381 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 1441 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 1501 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 1561 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 1621 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 1681 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 1741 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 1801 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 1861 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA 1921 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 1981 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 2041 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 2101 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 2161 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 2221 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 2281 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 2341 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 2401 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 2461 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 2521 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 2581 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC 2641 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 2701 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC 2761 TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA G //