LOCUS Exported 2822 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2822 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 859..906 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2754..2770 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2771..2802 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..727 /label="amilRFP" /note="" primer_bind complement(911..927) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(994..1582) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1773..2633) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2634..2738) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 762..821 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGCGCTGTCC AAACACGGCT TGACTAAAGA 61 TATGACGATG AAATACCACA TGGAAGGTTC AGTTGACGGT CACAAGTTCG TTATTACCGG 121 GCACGGTAAC GGTAACCCAT TCGAAGGTAA ACAAACCATG AACCTGTGCG TGGTAGAAGG 181 TGGTCCGTTG CCGTTCAGCG AGGACATTCT GTCGGCGGCT TTCGATTACG GTAACCGTGT 241 GTTCACCGAA TACCCGCAGG GTATGGTTGA TTTCTTCAAA AACTCCTGCC CGGCCGGTTA 301 CACTTGGCAC CGTTCTCTGC TTTTCGAAGA TGGTGCGGTG TGCACTACCT CGGCAGATAT 361 TACGGTTTCG GTGGAGGAAA ACTGCTTCTA TCATAACTCT AAGTTCCATG GCGTGAACTT 421 TCCGGCCGAC GGTCCAGTTA TGAAGAAAAT GACGACCAAC TGGGAACCAT CCTGCGAAAA 481 GATTATCCCG GTTCCGCGTC AGGGCATTCT GAAGGGCGAC ATCGCTATGT ACCTGCTGCT 541 GAAGGACGGT GGTCGTTACC GTTGCCAGTT TGACACCATT TATAAAGCTA AAAGCGACCC 601 GAAAGAAATG CCGGAATGGC ACTTTATTCA GCACAAACTG ACGCGTGAGG ACCGCAGTGA 661 TGCGAAAAAC CAAAAATGGC AACTCGTGGA GCACGCTGTC GCATCTCGCT CCGCACTGCC 721 GGGCTGAAGA GCTTTGAGAC CCAACCTGGT ATGCAGTCCT TCTAGATTAG CACTCCATAG 781 ACTAGGGATA ACAGGGTAAT CGATACCAAT GATTTCGATG ACGCTGAGGT GTCAATCGTC 841 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT 901 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA 961 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA 1021 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 1081 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1141 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 1201 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 1261 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1321 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 1381 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 1441 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 1501 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 1561 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT 1621 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 1681 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 1741 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 1801 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 1861 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA 1921 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 1981 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 2041 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 2101 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 2161 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 2221 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 2281 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 2341 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 2401 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 2461 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 2521 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 2581 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 2641 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 2701 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC 2761 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG 2821 AG //