LOCUS Exported 2828 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2828 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 865..912 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2760..2776 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2777..2808 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..733 /label="meffRed/meffRFP Biobricks K592012" /note="" primer_bind complement(917..933) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1000..1588) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1779..2639) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2640..2744) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 768..827 /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." /label="FreeGenes Barcode" ORIGIN 1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGCACTGAGT AAAAATGGCC TGACCAAAAA 61 CATGACGACC AAATATCGTA TGGAAGGTTG CGTGGACGGT CACAAGTTCG TTATCACCGG 121 CGATGGTATT GGCGACCCGT TTGAAGGTAA ACAGACGAGC ATCGATCTGT GCGTGGTTGA 181 AGGCGGTCCG CTGCCGTTCA GTGAAGATAT TCTGTCCGCA GTTTTTGACT ATGGCAACCG 241 TGTTTTCACC AAATATCCGC AGGATCTGGT TGACTACTTT AAGAATAGCT GTCCGGCGGG 301 TTACACCTGG CAACGCTCTT TTCTGTTCGA GGACGGTGCA GTCTGCACCG CGTCAGCCGA 361 TATTACGGTT TCGGTGGAAG AAAACTGTTT CTACCATGAA AGCAAGTTTC ACGGTGTGAA 421 TTTCCCGGCC GATGGCCCGG TTATGAAAAA GATGACCACG AATTGGGAAC CGTCTTGCGA 481 AAAAATTACC CCGATCCCGA ATGAAGGTAT CCTGAAAGGC GACGTCACGA TGTTTCTGCT 541 GCTGAAGGAT GGCGGTCGTT ATCGCTGTCA ATTCGATACC GTGTACAAAG CAAAGAGTGA 601 CCCGAAAACG ATTATGATGC CGGATTGGCA TTTTATCCAG CACAAGCTGA ACCGTGAAGA 661 TCGCTCCGAC GCTAAACATC AAAAGTGGCG TCTGGTGGAA AATGCAATCG CTTACCGCAG 721 CACCCTGTCC TGAAGAGCTT TGAGACCGGC GGTGTCTACA TATCCTTTCA ATCGCTCTCG 781 ACCGGCGATA GGGATAACAG GGTAATAGAG CGACTCTTCC CCGGTAACGC TGAGGTGTCA 841 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG 901 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT 961 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC 1021 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 1081 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 1141 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 1201 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 1261 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 1321 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 1381 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 1441 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 1501 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 1561 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG 1621 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 1681 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 1741 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 1801 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 1861 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 1921 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 1981 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 2041 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC 2101 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA 2161 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG 2221 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT 2281 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC 2341 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG 2401 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG 2461 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT 2521 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA 2581 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA 2641 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 2701 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG 2761 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG 2821 AGTGGGAG //