LOCUS       Exported     2816 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2816
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      853..900
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2748..2764
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2765..2796
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..721
                     /label="scubRFP"
                     /note=""
     primer_bind     complement(905..921)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(988..1576)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1767..2627)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2628..2732)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    756..815
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GTCTGCGATC AAAACCGTTG TTAAACAGTT
       61 CATGAAAATC AAAATGTCTC TGGAGGGTAC TGTCAACGGC CACTACTTCA AAATTGTCGG
      121 TGAGGGCGAC GGCACCCCGT TCGAGGGTAA ACAGACTCTT CATCTGAAAG TCAAAGAAGG
      181 AGCCCCGCTG CCTTTTGCTT ACGATATTTT AACCACTGCG CTGCATTACG GGAACCGTGT
      241 TTTCGTCGAA TACCCGGAGA ACATTCCAGA CTATTTCAAA CAGTCGTTCC CGAAAGGTTA
      301 CTCTTGGGAA CGCAGCCTGA CCTTCGAAGA TGGTGGCATC TGTATCGCCC GTTCTGACAT
      361 CAAGATGGTG GGCGATACCT TCCATAACGA AGTTCAGTTC TATGGCGTTA ACTTCCCGGC
      421 GAACGGTCCG GTTATGCAGC GTCACACCGT TAAATGGGAG CCGAGCACCG AGAAAATCTA
      481 CGTTCGTGAC GGTGTGCTGA CCGGTGATAT TACTATGGCA CTGCTGCTCA AAGGTGGTAC
      541 TCACTACCGT TGCGACTTCC GCACCACCTA TAAAGCGAAA GAAAAAGGTC CGAAATTCCC
      601 GGGTTACCAC CTGGTGGATC ACTGTATCGA GATTACTTCT CACGATAAAG ATTATAACGT
      661 GGTTGAACTG TACGAACACG CTGTGGCTCA CTCCGGTCTG CCGGACTCCG CGAACCGCTG
      721 AAGAGCTTTG AGACCGCGGT CGCTATACAT ACCTTTTGCC CCGCTTAAAC TATACCTAGG
      781 GATAACAGGG TAATCCGTTG GCTCAGCGTC GATGCCGCTG AGGTGTCAAT CGTCGGAGCC
      841 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
      901 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
      961 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1021 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1081 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1141 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1201 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1261 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1321 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1381 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1441 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1501 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1561 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     1621 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     1681 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     1741 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     1801 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     1861 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     1921 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     1981 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2041 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2101 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2161 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2221 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2281 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2341 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2401 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2461 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2521 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     2581 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     2641 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     2701 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     2761 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//