LOCUS       Exported     2825 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2825
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      862..909
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2757..2773
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2774..2805
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..730
                     /label="EiraCFP Biobrick J97000"
                     /note=""
     primer_bind     complement(914..930)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(997..1585)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1776..2636)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2637..2741)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    765..824
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GTCGTCTGGT ACCAAATTGT TTGAAAAGGA
       61 AATCCCGTAT ATCACTGAAC TGGAGGGCGA CGTCAATGGT ATGAAGTTTA CCATTCATGG
      121 TAAAGGTACC GGCGATGCGA GCACGGGTAC CATTAAAGCG AAATTCATCT GCACTACGGG
      181 CGACGTTCCG GTCCCGTGGG CAACCCTGGT GAGCACCCTG AGCTACGGTG TTCAGTGTTT
      241 CGCCAAGTAC CCGCCGCACA TCAAGGATTT CTTTAAGAGC GCCATGCCGG AAGGTTATGT
      301 TCAAGAGCGT ACCATCACCT TCGAAGGCGA CGGCGTGTTT AAGACGCGTG CTGAGGTTAC
      361 CTTTGAAAAC GGTTCTGTCT ACAATCGTGT CACGCTGAAC GGCCAGGGTT TTAAGAAAGA
      421 CGGTCACATT CTGCGTAAGA ACGTTGCATT CCAATGCCCG CCAGATATTG TGTATATTTT
      481 GCCTGACACC GTTAACAATG GCATCCGCGT TGAGTTCAAC CAGGCGTACG ATATTGAAGG
      541 TGTGACCGAA AAACTGGTTA CCAAATGCAG CCAAATGAAT CGTCCGTTGG CGGGCTCCGC
      601 GGCAGTGCAT ATCCCGCGTT ATCATCACCT GAGCAAACAC ACCAAACTGA GCAAAGACCG
      661 CGACGAGCGC CGTGATCACA TGTGTCTGGT AGAGGTCGTG AAAGCGGTTG ATCTGGACAC
      721 GTATCAGTGA AGAGCTTTGA GACCCGCGCT AATAGCTTTG CATTGCCTGC GTATATCTTT
      781 CTTTGTAGGG ATAACAGGGT AATGTACCAT TGAAAAGCTC GCTTCGCTGA GGTGTCAATC
      841 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
      901 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
      961 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1021 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1081 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1141 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1201 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1261 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1321 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1381 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1441 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1501 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1561 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     1621 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     1681 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     1741 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     1801 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     1861 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     1921 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     1981 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2041 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2101 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2161 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2221 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2281 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2341 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2401 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2461 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2521 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2581 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     2641 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     2701 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     2761 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT
     2821 GGGAG
//