LOCUS       Exported     3185 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3185
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1222..1269
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3117..3133
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3134..3165
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1090
                     /note=""
                     /label="horseradish peroxidase (HRP)"
     primer_bind     complement(1274..1290)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1357..1945)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2136..2996)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2997..3101)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1125..1184
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GCACTTCAGC TCTAGCAGTA CCCTGTTTAC
       61 CTGTATCACC CTGATCCCGC TGGTATGCCT GATCCTGCAT GCGAGCCTGA GCGACGCGCA
      121 GCTGACTCCG ACCTTCATCG ACAACTCCTG CCCGAACGTC AGTAACATCG TGCGTGACAC
      181 CATCGTCAAC GAACTGCGCT CCGATCCGCG TATCGCAGCT TCCATCCTGC GTCTGCACTT
      241 CCATGACTGT TTCGTGAACG GGTGCGATGC GTCTATTCTG CTGGATAACA CCACCTCTTT
      301 TCGTACTGAA AAAGATGCGT TCGGTAATGC TAACTCCGCA CGTGGCTTTC CGGTGATCGA
      361 TCGCATGAAA GCTGCCGTCG AGTCTGCGTG TCCGCGTACT GTTTCTTGCG CGGACCTGCT
      421 GACCATCGCC GCTCAGCAGT CCGTTACTCT GGCTGGTGGT CCGTCTTGGC GCGTTCCACT
      481 GGGTCGTCGT GATAGCCTGC AAGCATTTCT GGACCTGGCG AACGCGAATC TCCCGGCTCC
      541 GTTCTTCACC CTGCCGCAGC TGAAGGACTC TTTCCGCAAC GTTGGTCTGA ACCGTTCTTC
      601 TGACCTGGTG GCGCTGAGCG GCGGCCATAC GTTTGGCAAA AACCAGTGTC GTTTCATCAT
      661 GGACCGCCTG TATAACTTCA GCAACACGGG CCTGCCGGAC CCGACCCTCA ACACTACGTA
      721 CCTCCAAACC CTGCGTGGGC TGTGCCCGCT GAACGGTAAC TTATCCGCTC TGGTTGACTT
      781 CGATCTGCGT ACCCCGACTA TCTTCGATAA CAAGTACTAC GTTAATCTGG AAGAACAGAA
      841 AGGTCTGATC CAGTCCGACC AGGAACTGTT TTCTTCTCCG AACGCGACCG ATACCATCCC
      901 GCTGGTGCGG TCGTTTGCTA ACTCCACCCA GACTTTCTTT AACGCCTTCG TAGAGGCTAT
      961 GGATCGTATG GGCAATATCA CTCCACTGAC TGGCACTCAG GGCCAGATCC GTCTGAACTG
     1021 TCGTGTCGTT AACTCAAACA GCCTGCTCCA CGACATGGTT GAAGTGGTTG ACTTTGTCTC
     1081 TTCTATGTGA AGAGCTTTGA GACCGACGAC TGCCTAAGGA CATTCCGGAC ATGTCGGAGG
     1141 AAGAATAGGG ATAACAGGGT AATGCTGCAC TTTCCGGGCT TCAACGCTGA GGTGTCAATC
     1201 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1261 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1321 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1381 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1441 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1501 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1561 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1621 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1681 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1741 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1801 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1861 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1921 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     1981 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2041 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2101 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2161 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2221 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2281 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2341 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2401 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2461 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2521 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2581 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2641 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2701 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2761 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2821 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2881 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2941 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3001 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3061 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3121 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT
     3181 GGGAG
//