LOCUS       Exported     3776 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3776
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1813..1860
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3708..3724
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3725..3756
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1681
                     /label="Firefly Luciferase [Photinus pyralis] (Fluc)"
                     /note=""
     primer_bind     complement(1865..1881)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1948..2536)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2727..3587)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3588..3692)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1716..1775
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGAAGATGCG AAGAACATCA AAAAAGGCCC
       61 TGCACCGTTC TATCCGCTGG AAGATGGTAC AGCTGGTGAG CAACTGCATA AAGCCATGAA
      121 ACGCTATGCA CTGGTTCCGG GCACCATCGC CTTCACCGAT GCCCACATCG AAGTTAACAT
      181 TACGTACGCA GAGTATTTCG AAATGTCTGT TCGTTTAGCC GAAGCGATGA AACGTTATGG
      241 CCTGAATACT AACCACCGCA TTGTAGTGTG CAGCGAGAAC TCCCTGCAAT TCTTCATGCC
      301 GGTGCTGGGC GCTCTGTTCA TCGGCGTGGC TGTTGCACCA GCTAACGACA TCTACAACGA
      361 GCGTGAACTG CTGAACTCTA TGAATATCTC TCAGCCGACA GTAGTTTTCG TTAGCAAAAA
      421 AGGCCTGCAA AAAATCTTAA ACGTACAGAA GAAACTGCCT ATCATTCAGA AAATCATCAT
      481 TATGGACTCT AAAACCGATT ATCAGGGCTT CCAGTCGATG TATACCTTCG TTACTAGCCA
      541 CCTCCCGCCG GGTTTCAACG AGTACGATTT CGTGCCAGAA AGCTTCGACC GCGATAAAAC
      601 CATCGCGCTG ATTATGAATA GCTCTGGTTC CACTGGTCTG CCGAAAGGTG TAGCACTGCC
      661 CCATCGTACC GCATGCGTTC GTTTCTCTCA CGCTCGTGAT CCGATCTTTG GCAACCAGAT
      721 CATTCCGGAT ACGGCTATCC TGAGCGTTGT TCCGTTCCAC CATGGCTTTG GTATGTTCAC
      781 CACCCTGGGT TACCTGATCT GCGGTTTCCG TGTTGTCCTG ATGTACCGCT TTGAAGAAGA
      841 ACTGTTCCTG CGTTCCCTGC AAGACTACAA AATCCAGTCT GCCTTACTCG TTCCGACCCT
      901 GTTCAGCTTT TTCGCCAAAT CTACCCTGAT CGATAAGTAC GATCTGTCCA ATCTGCACGA
      961 AATCGCGTCC GGCGGTGCAC CGCTGTCTAA AGAAGTTGGT GAAGCAGTAG CTAAACGTTT
     1021 CCACCTGCCG GGTATCCGTC AGGGCTATGG TCTGACCGAA ACTACCTCTG CAATCCTGAT
     1081 CACCCCAGAG GGTGACGATA AGCCGGGTGC TGTTGGTAAA GTAGTGCCTT TCTTCGAAGC
     1141 AAAGGTTGTC GACCTGGACA CTGGCAAAAC GCTGGGTGTT AACCAGCGCG GCGAACTGTG
     1201 TGTTCGCGGC CCGATGATTA TGAGCGGCTA TGTCAACAAC CCGGAAGCGA CCAACGCGCT
     1261 GATCGATAAA GATGGCTGGC TGCACTCTGG TGACATCGCC TACTGGGACG AGGACGAACA
     1321 CTTCTTTATC GTAGATCGTC TGAAGTCTCT GATCAAGTAC AAGGGTTACC AAGTGGCACC
     1381 GGCCGAACTG GAAAGCATCC TGCTGCAACA CCCCAACATC TTCGACGCAG GCGTCGCAGG
     1441 CCTGCCGGAC GACGATGCAG GTGAACTGCC GGCGGCGGTT GTAGTCCTGG AACATGGCAA
     1501 GACCATGACC GAGAAAGAAA TCGTGGACTA TGTCGCGTCC CAAGTCACCA CCGCGAAGAA
     1561 ACTGCGCGGT GGTGTTGTAT TCGTGGACGA AGTGCCTAAA GGTCTGACCG GCAAACTGGA
     1621 TGCGCGCAAA ATTCGTGAAA TCCTGATCAA AGCGAAAAAA GGTGGCAAAT CCAAACTGTG
     1681 AAGAGCTTTG AGACCCTATT CGGTCTGCTC GTTGTAACTA CGGCGGCGTG CTGTTGTAGG
     1741 GATAACAGGG TAATTACCTC TCGATCGTTC TTGCCCGCTG AGGTGTCAAT CGTCGGAGCC
     1801 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1861 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1921 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1981 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     2041 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     2101 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     2161 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     2221 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     2281 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     2341 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     2401 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     2461 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2521 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     2581 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     2641 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     2701 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     2761 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     2821 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     2881 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2941 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     3001 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     3061 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     3121 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     3181 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     3241 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     3301 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     3361 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     3421 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     3481 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     3541 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     3601 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     3661 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     3721 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//