LOCUS       Exported     3770 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3770
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1807..1854
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3702..3718
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3719..3750
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1675
                     /label="Luciferase [Luciola cruciata] (Japanese firefly)"
                     /note=""
     primer_bind     complement(1859..1875)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1942..2530)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2721..3581)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3582..3686)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1710..1769
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGAAAATATG GAAAACGATG AAAACATCGT
       61 AGTGGGCCCG AAACCATTCT ATCCGATCGA AGAAGGCTCC GCCGGCACCC AGCTGCGCAA
      121 ATACATGGAA CGTTACGCAA AGCTCGGTGC AATCGCTTTC ACCAACGCAG TGACCGGTGT
      181 TGACTACTCT TATGCGGAGT ACCTGGAAAA ATCCTGCTGC CTCGGCAAGG CGCTGCAAAA
      241 CTATGGCCTC GTAGTTGACG GTCGTATTGC TCTGTGTAGT GAAAACTGCG AAGAGTTCTT
      301 CATCCCGGTT ATCGCTGGTC TGTTCATTGG TGTGGGTGTT GCTCCTACCA ACGAAATCTA
      361 CACCCTGCGC GAACTCGTTC ACTCCCTGGG TATCAGCAAA CCGACCATTG TTTTCTCTTC
      421 TAAAAAAGGC CTGGATAAAG TGATCACCGT TCAGAAAACG GTAACTACTA TCAAGACCAT
      481 TGTTATCCTG GATAGTAAAG TCGACTATCG CGGTTATCAG TGCCTGGACA CCTTCATCAA
      541 ACGTAACACT CCGCCGGGTT TCCAGGCTTC TTCCTTTAAA ACTGTCGAGG TCGACCGCAA
      601 AGAACAGGTG GCTCTGATCA TGAACAGTTC CGGCTCTACC GGGCTGCCGA AAGGCGTTCA
      661 GCTGACCCAC GAAAACACTG TGACTCGTTT CTCCCACGCA CGTGACCCCA TCTACGGCAA
      721 CCAGGTGAGC CCGGGCACCG CCGTGCTCAC CGTTGTGCCG TTCCACCACG GTTTCGGCAT
      781 GTTCACTACC CTGGGCTACC TGATTTGCGG TTTCCGTGTT GTAATGCTGA CTAAATTCGA
      841 TGAGGAAACC TTCTTGAAAA CCCTTCAGGA TTATAAATGT ACATCCGTGA TCCTGGTTCC
      901 GACTCTGTTC GCTATCCTGA ACAAATCTGA ACTGCTTAAC AAATACGACC TGTCCAACCT
      961 GGTTGAGATC GCAAGCGGTG GTGCTCCGCT GTCTAAAGAA GTAGGTGAAG CTGTTGCCCG
     1021 TCGTTTCAAC CTGCCGGGTG TGCGTCAGGG CTATGGTCTG ACGGAAACCA CTTCTGCGAT
     1081 TATCATCACT CCGGAAGGTG ACGACAAACC GGGTGCGTCT GGCAAAGTGG TTCCTCTCTT
     1141 CAAAGCTAAG GTCATTGACT TGGATACCAA GAAATCTCTG GGCCCGAACC GTCGTGGTGA
     1201 AGTCTGTGTT AAAGGCCCGA TGCTGATGAA AGGCTACGTG AACAACCCAG AAGCGACCAA
     1261 GGAGTTGATT GACGAGGAGG GCTGGCTGCA CACCGGGGAT ATCGGCTATT ATGATGAGGA
     1321 GAAACACTTT TTTATCGTTG ATCGTTTGAA GTCCCTGATC AAATACAAAG GTTATCAGGT
     1381 GCCTCCGGCG GAACTGGAAT CTGTACTGCT GCAACACCCG TCAATTTTCG ACGCCGGCGT
     1441 GGCTGGTGTA CCGGACCCGG TAGCCGGTGA ACTGCCAGGA GCTGTCGTTG TGCTGGAATC
     1501 GGGCAAAAAC ATGACTGAAA AAGAAGTGAT GGACTATGTC GCTTCTCAGG TTAGTAACGC
     1561 TAAGCGCCTG CGCGGCGGCG TTCGCTTCGT TGATGAAGTA CCGAAAGGCC TGACCGGCAA
     1621 AATCGATGGC CGCGCTATCC GTGAAATCCT GAAAAAACCG GTTGCTAAAA TGTGAAGAGC
     1681 TTTGAGACCG TGATATGCCG ACTCCTTGTC CAGCCGACAT GTGCAGACCA TAGGGATAAC
     1741 AGGGTAATAT ATCTTGTGAG TAACTAACCC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1801 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1861 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1921 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1981 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     2041 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     2101 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     2161 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     2221 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     2281 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     2341 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     2401 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     2461 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     2521 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     2581 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     2641 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     2701 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2761 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2821 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2881 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2941 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     3001 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     3061 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     3121 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     3181 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     3241 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     3301 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     3361 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     3421 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     3481 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     3541 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     3601 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3661 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3721 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
//