LOCUS       Exported     3059 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3059
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1096..1143
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2991..3007
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3008..3039
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..964
                     /label="Renilla luciferase"
                     /note=""
     primer_bind     complement(1148..1164)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1231..1819)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2010..2870)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2871..2975)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    999..1058
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GACCTCCAAA GTGTACGATC CGGAACAGCG
       61 TAAGCGTATG ATCACTGGTC CGCAGTGGTG GGCCCGCTGT AAACAGATGA ACGTTCTGGA
      121 CAGCTTCATC AACTACTACG ATTCCGAAAA ACACGCAGAG AATGCTGTTA TCTTCCTGCA
      181 CGGCAACGCA GCCAGCTCTT ACCTGTGGCG TCATGTTGTT CCACATATCG AACCAGTGGC
      241 GCGCTGCATT ATCCCGGATC TGATTGGTAT GGGCAAAAGC GGTAAATCCG GTAACGGTAG
      301 CTATCGTCTG CTCGACCATT ACAAGTATCT GACCGCGTGG TTCGAACTCC TCAATCTGCC
      361 GAAAAAAATC ATCTTTGTCG GGCACGATTG GGGTGCTTGC CTGGCCTTCC ACTATTCTTA
      421 CGAACACCAG GATAAAATCA AAGCGATCGT ACATGCAGAA TCCGTGGTGG ACGTGATCGA
      481 GTCTTGGGAT GAGTGGCCGG ATATCGAAGA AGATATTGCT CTGATTAAAT CTGAAGAAGG
      541 TGAGAAAATG GTACTGGAAA ACAACTTCTT CGTAGAAACC ATGCTGCCGT CTAAAATTAT
      601 GCGCAAACTG GAGCCGGAAG AATTTGCTGC GTATTTGGAA CCGTTCAAGG AAAAGGGCGA
      661 AGTACGTCGT CCAACTCTGA GCTGGCCTCG TGAAATCCCG CTTGTGAAAG GCGGTAAACC
      721 GGATGTCGTG CAGATTGTCC GTAACTACAA CGCCTATCTG CGCGCTTCCG ATGATCTGCC
      781 GAAAATGTTT ATCGAATCTG ACCCAGGTTT CTTCTCCAAC GCGATCGTAG AAGGCGCCAA
      841 AAAGTTCCCG AACACCGAAT TTGTGAAGGT AAAAGGTCTG CACTTCTCTC AGGAAGATGC
      901 GCCGGATGAA ATGGGTAAGT ACATTAAAAG CTTCGTAGAA CGTGTGTTGA AGAACGAACA
      961 GTGAAGAGCT TTGAGACCGT AGTGCCTGTT CGTTCTGTAT CTGATTCCCT GGCTTTATTT
     1021 AGGGATAACA GGGTAATTAT TATGCTTCGC TGAATGTGCG CTGAGGTGTC AATCGTCGGA
     1081 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1141 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1201 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1261 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1321 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1381 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1441 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1501 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1561 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1621 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1681 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1741 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1801 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1861 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1921 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1981 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2041 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2101 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2161 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2221 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2281 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2341 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2401 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2461 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2521 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2581 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2641 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2701 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2761 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2821 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2881 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2941 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3001 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//