LOCUS Exported 3716 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3716 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1753..1800 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3648..3664 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3665..3696 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1621 /label="Calf Intestine Alkaline phosphatase (CIAP)" /note="" primer_bind complement(1805..1821) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1888..2476) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2667..3527) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3528..3632) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1656..1715 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AACGATGATG CTCACTCTCG GGTCTCTAAT GCAATGGGCC TGCGTTCTGC TGCTGCTGGG 61 CCTGTGGCTC CAGCTGTCCC TGACCTTCAT CCCTGCCGAA GAAGAGGACC CGGCGTTCTG 121 GAATCGTCAG GCAGCGCAGG CTCTCGACGT AGCTAAAAAA CTGCAACCAA TCCAGACCGC 181 AGCGAAAAAC GTGATCCTGT TCCTGGGTGA CGGCATGGGT GTTCCTACCG TTACCGCAAC 241 CCGTATCCTG AAAGGCCAGA TGAACGGCAA ACTCGGCCCA GAAACCCCTC TGGCTATGGA 301 TCAATTCCCG TACGTGGCGC TGAGCAAAAC CTACAACGTT GACCGCCAAG TTCCGGACTC 361 TGCGGGCACC GCGACTGCTT ATTTGTGTGG GGTTAAAGGC AACTACAAAA CTATCGGTGT 421 TTCTGCGGCA GCTCGTTATA ACCAGTGCAA CACCACCTCA GGTAACGAGG TAACCAGCGT 481 GATGAACCGT GCTAAAAAGG CCGGTAAATC CGTAGGCGTT GTTACGACCT CCCGTGTTCA 541 GCATGCAAGC CCGGCGGGTG CATACGCGCA CACTGTCAAC CGTAACTGGT ACAGCGATGC 601 GGATCTGCCG GCGGATGCAC AGACCTACGG TTGCCAGGAC ATCGCAACCC AGCTGGTTAA 661 TAACATGGAC ATCGACGTTA TCCTGGGTGG CGGTCGTATG TACATGTTCC CGGAAGGCAC 721 CCCGGACCCA GAATACCCGT ACGACGTAAA CCAGACAGGT GTCCGTAAGG ACAAACGTAA 781 CTTGGTCCAG GAGTGGCAAG CGAAGCACCA GGGTGCTCAG TACGTGTGGA ACCGTACCGA 841 ATTGCTGCAA GCTGCGAATG ATCCGTCCGT TACGCACCTG ATGGGCCTCT TTGAACCTGC 901 CGACATGAAA TATAACGTAC AGCAAGATCC GACTAAAGAC CCGACTCTGG AGGAAATGAC 961 TGAGGCTGCA CTTCAGGTGC TGTCTCGTAA CCCGCAGGGT TTCTACCTGT TTGTAGAAGG 1021 TGGCCGTATT GACCATGGTC ACCACGAGGG TAAGGCATAC ATGGCCCTGA CCGATACCGT 1081 CATGTTCGAT AACGCCATCG CCAAAGCAAA CGAACTGACA TCAGAACTGG ATACCCTGAT 1141 CCTGGCAACC GCTGATCACT CCCACGTGTT CTCTTTCGGC GGTTACACGC TTCGCGGTAC 1201 CTCCATCTTC GGTCTGGCTC CATCCAAAGC GTCTGATAAC AAGTCTTACA CCTCCATCCT 1261 GTATGGTAAC GGGCCGGGCT ACGTGCTGGG TGGTGGTCTG CGTCCGGATG TTAACGACTC 1321 GATCAGCGAA GATCCGAGCT ACCGTCAGCA GGCGGCGGTG CCTCTGTCCA GTGAATCTCA 1381 CGGCGGCGAA GATGTGGCGG TTTTTGCACG TGGCCCACAG GCACACTTAG TCCACGGTGT 1441 GCAGGAAGAA ACCTTTGTCG CGCACGTTAT GGCTTTTGCA GGCTGCGTTG AACCGTACAC 1501 TGACTGTAAC CTGCCGGCAC CGTCTGGCCT GAGCGACGCC GCACACCTGG CTGCCTCTCC 1561 GCCGAGCCTG GCCCTGCTGG CAGGTGCTAT GCTGCTGCTT CTGGCGCCGG CTCTGTACTG 1621 AAGAGCTTTG AGACCGGGCA CTCAATATAC CCTGTTGTGC TCCGGTTTCT GCCCTGTAGG 1681 GATAACAGGG TAATGGTGAC GCCTCGGACG TGCTCCGCTG AGGTGTCAAT CGTCGGAGCC 1741 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG 1801 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT 1861 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 1921 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 1981 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 2041 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 2101 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 2161 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 2221 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 2281 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 2341 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 2401 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 2461 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG 2521 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 2581 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 2641 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 2701 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 2761 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA 2821 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 2881 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 2941 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 3001 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 3061 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 3121 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 3181 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 3241 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 3301 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 3361 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 3421 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 3481 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 3541 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 3601 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC 3661 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG //