LOCUS       Exported     3027 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3027
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1064..1111
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2959..2975
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2976..3007
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="AE_spisPink pDest"
                     /note=""
     primer_bind     complement(1116..1132)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1199..1787)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1978..2838)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2839..2943)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="AE_spisPink pDest"
                     /note=""
     misc_feature    967..1026
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGAGCGAGAC CATTGACAGC GCAGCTGGCA CGACAGGTTT
       61 CCCGACTGGA AAGCGTTGAC AGCTAGCTCA GTCCTAGGTA TAATGCTAGC TACTAGAGAA
      121 AGAGGAGAAA TACTAAATGT CGCACTCAAA ACAAGCACTG GCAGATACGA TGAAAATGAC
      181 GTGGCTGATG GAAGGTTCGG TCAACGGTCA CGCCTTTACG ATTGAAGGCG AAGGTACCGG
      241 CAAACCGTAT GAAGGTAAAC AGAGTGGCAC CTTTCGTGTT ACGAAAGGCG GTCCGCTGCC
      301 GTTTGCGTTT GATATTGTCG CTCCGACCCT GAAATACGGT TTCAAATGCT TCATGAAATA
      361 CCCGGCGGAT ATTCCGGACT ACTTTAAACT GGCCTTCCCG GAAGGCCTGA CGTACGATCG
      421 TAAAATTGCG TTTGAGGACG GCGGTTGTGC GACCGCCACG GTCGAAATGA GCCTGAAGGG
      481 TAACACCCTG GTGCATAAAA CGAACTTTCA GGGCGGTAAT TTCCCGATTG ATGGTCCGGT
      541 GATGCAAAAA CGCACCCTGG GCTGGGAACC GACCTCTGAA AAAATGACGC CGTGCGATGG
      601 TATTATCAAA GGCGACACCA TCATGTACCT GATGGTTGAA GGCGGTAAAA CGCTGAAATG
      661 TCGTTATGAA AACAATTACC GCGCCAACAA ACCAGTGCTG ATGCCGCCGA GCCACTTTGT
      721 GGATCTGCGT CTGACCCGCA CGAATCTGGA TAAAGAAGGT CTGGCGTTCA AACTGGAAGA
      781 ATATGCTGTT GCCCGTGTGC TGGAAGTGTA ATAAGCTTCA AATAAAACGA AAGGCTCAGT
      841 CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC TACTAGAGTC
      901 ACACTGGCTC ACCTTCGGGT GGGCCTTTCT GCGCGGTCTC AGCTTACGTG AATCTTGACC
      961 TGTGGTCTAG GAATCTGAGT CCGCCGATAG GGATAACAGG GTAATGGGAC TTCCACTAGA
     1021 TAGAACCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
     1081 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG
     1141 GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT
     1201 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
     1261 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
     1321 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
     1381 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
     1441 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
     1501 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
     1561 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
     1621 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
     1681 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
     1741 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT
     1801 TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     1861 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     1921 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     1981 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     2041 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     2101 TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     2161 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     2221 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
     2281 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
     2341 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
     2401 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
     2461 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
     2521 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
     2581 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
     2641 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
     2701 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
     2761 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
     2821 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
     2881 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
     2941 GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT
     3001 TTTGACTTGG TTCAGGTGGA GTGGGAG
//