LOCUS       Exported     5649 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..5649
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      3686..3733
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     5581..5597
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      5598..5629
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..3553
                     /label="AB_Dawn "
                     /note=""
     primer_bind     complement(3738..3754)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(3821..4409)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(4600..5460)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(5461..5565)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3589..3648
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAGGA GAGAAGACAG TCATAAGTGC GGCACGATAG
       61 TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC
      121 GAGATCCCGG TGCCTAATGA GTGAGCTAAC TTACATCAAT TGTCAATCGT TGAGCATGCC
      181 GGCGCGCATC GCGAGGCGAA CCAGCTCCGA AAGGCTGTTG GCCTGCATCT TGGTCATGAC
      241 GTTGGCCCGA TACACCTCGA TGGTGCGCGG GCTGATGTCG TACTCGCGGG CGATCAGCTT
      301 GTTGGAAAGG CCGGCGATCA GCCCTTCCAT GACCTGGCGC TCCCTGGGGC TCAACGAGGC
      361 GACGCGGGCG GCGATATCCT GCGCGACGGC CTCGCTCTTG GCGGCCGGCT CGGCCTGGCG
      421 GATCGCCGAT TCGATCATGG CGGTGAGGCG GTCGTCCTCG AAAGGCTTTT CCAGAAAGTC
      481 CACCGCCCCT AACTTCATCG CCTCGACCGC GAGCGGCACG TCGCCGTGAC CGGTCATGAT
      541 GAGGATCGGA AAGGGGCTTT GCTGCGCCTT CATCCGCTTC AACAGCTCGA TGCCGTCAAG
      601 GCCCGGCATG CGCACGTCGG ACACGACGCA GCCGAAGGAC AGACCCGGCA GGGCGTCGAG
      661 AAAGGCTTGC GCGTCGTCAA ACAGCGTGAC GCCGAAGCCG GCAGAATCCA GCAGGAAATT
      721 CAGCGAATCC CGCATCGCCG CGTCGTCGTC GATGACGTAG ATATGTCCCT TGGTCGTCAT
      781 GCTAGACCTC CTATCATCTC GTCGGCTGCC GGGAGGGTGA AGCGGAAGGT CGCCCCGCCC
      841 GATGCGTTGC TCTCGGCCCA CATGCGCCCG CCGTGAGCTT CGATGATCGA GCGGCTGATG
      901 GACAGTCCCA CGCCCATGCC GGTGTCCTTG GTGGTGAAGA AAGTCTGAAA CAGGTTCGGA
      961 ATGACGTCGT CCTGGAAACC GCTGCCGGTG TCGGACACTT CGACCTCGAT CATGTCGTCG
     1021 GCGGCGGGGG TGTTGGTGAC GACGAGCTCG CGTCGCTGCG ACTGAGCCAT CGCTTCCAGC
     1081 GCGTTGCGGA ACAGGTTGAC CAGGACCTGC TGGATCTGCA CCCGGTCGGC GAGAACGAGA
     1141 TCGGCGCCCG GATCGAGACT GAAGCGGAGC TGCACGTTCT GCTCGCGCGC GCCGGCAAGC
     1201 CCGAGCGCGC CGGCCTCCTC GATCAGCTTG GAGAGACTCT CGACCCGCTT CTCCGATTCG
     1261 CCGCGGGCAA CGAAGTCGCG CAGGCGCCGG ATGATCTGGC CGGCGCGCAG CGCCTGCTCG
     1321 GCGGCGCGGT CCAGGGCGCT TTCGACCTTC GGTGTGTTCG GATCACTGCT GCCGGCAAGC
     1381 AGCCGCCGCG AGCCCTTCAT GTAGTTGCTG ATCGCCGCCA GCGGCTGGTT GAGCTCGTGC
     1441 GCGAGCGCGG ACGCCATTTC GCCCATGGCG CTCAGCCTGG AAACGTGGAC GAGCTCGGAT
     1501 TGCAGTTCCT GGAGGCGCGC CTGGGTCTGC TGGTGCTCGG TGATATCATT CTGAATACCG
     1561 ACAAAATACG TTTTATCCTC TATTTCCATT GGATCAATAT TTAATTCATT CCAGAACATC
     1621 GTTCCGTCTT TTTTGTAGTT TTGGATCTGA ACGGTGACCG GTTCTTTATT TTGTAAAGCG
     1681 GTTCTGATGT TGTCCACTTC TGCAGGATCT GTGTGTTTCC CCTGTAAGAA GCGACAGTTC
     1741 TTTCCTAAAA TTTCCTCGGT TTCGTAGCCG GTCATTTGAA CAAAGCCTTG ATTTACGTAG
     1801 ACAATAGGAT TATCTTCAAG TGCGGGATCT GTAATTACCA CACCGACTCG CACGTGATCA
     1861 AGTGCTTTTT TGATGACTTC CAGCTGTCCT GGTATCCCAA ATGATTGAAA ACTAGCCACA
     1921 TTCACCACCC TCAATTGACT CTCTTCCGGG CGCTATCATG CCATACCGCG AAAGGTTTTG
     1981 CACCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA
     2041 GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA
     2101 GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA
     2161 AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA
     2221 GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG
     2281 GATCGAGATC TACGCCCGTG ATCCTGATCA CCGGCTATCC GGACGAAAAC ATCTCGACCC
     2341 GGGCCGCCGA GGCCGGCGTA AAAGACGTGG TTTTGAAGCC GCTTCTCGAC GAAAACCTGC
     2401 TCAAGCGTAT CCGCCGCGCC ATCCAGGACC GGCCTCGGGC ATGACCTACG GGGTTCTACG
     2461 TAAGGCACCC CCCTTAAGAT ATCGCTCGAA ATTTTCGAAC CTCCCGATAC CGCGTACCAA
     2521 TGCGTCATCA CAACGGAGTC TAGAAAAGAG GAGAAATACT AGATGAGCAC AAAAAAGAAA
     2581 CCATTAACAC AAGAGCAGCT TGAGGACGCA CGTCGCCTTA AAGCAATTTA TGAAAAAAAG
     2641 AAAAATGAAC TTGGCTTATC CCAGGAATCT GTCGCAGACA AGATGGGGAT GGGGCAGTCA
     2701 GGCGTTGGTG CTTTATTTAA TGGCATCAAT GCATTAAATG CTTATAACGC CGCATTGCTT
     2761 GCAAAAATTC TCAAAGTTAG CGTTGAAGAA TTTAGCCCTT CAATCGCCAG AGAAATCTAC
     2821 GAGATGTATG AAGCGGTTAG TATGCAGCCG TCACTTAGAA GTGAGTATGA GTACCCTGTT
     2881 TTTTCTCATG TTCAGGCAGG GATGTTCTCA CCTGAGCTTA GAACCTTTAC CAAAGGTGAT
     2941 GCGGAGAGAT GGGTAAGCAC AACCAAAAAA GCCAGTGATT CTGCATTCTG GCTTGAGGTT
     3001 GAAGGTAATT CCATGACCGC ACCAACAGGC TCCAAGCCGA GCTTTCCTGA CGGAATGTTA
     3061 ATTCTCGTTG ACCCTGAGCA GGCTGTTGAG CCAGGTGATT TCTGCATAGC CAGACTTGGG
     3121 GGTGATGAGT TTACCTTCAA GAAACTGATC AGGGATAGCG GTCAGGTGTT TTTACAACCA
     3181 CTAAACCCAC AGTACCCAAT GATCCCATGC AATGAGAGTT GTTCCGTTGT GGGGAAAGTT
     3241 ATCGCTAGTC AGTGGCCGGA GGAAACGTTT GGCGCTGCAA ACGACGAAAA CTACGCTTTA
     3301 GTAGCTTAAT AACGCTGATA GTGCTAGTGT AGATCGCTAC TAGAGCCAGG CATCAAATAA
     3361 AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG
     3421 CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATATACTAG
     3481 AGTAACACCG TGCGTGTTGA CTATTTTACC TCTGGCGGTG ATAATGGTTG CTCTAGAAAT
     3541 AATTTTGTTT AACTTTTACT CGAGACCAGG GTGCCTTGCT AATCTAGTCT AATCCACAAT
     3601 CAAGAGAGCT AGGGATAACA GGGTAATCCG TGCCTCATTT GTCATTCACG CTGAGGTGTC
     3661 AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT
     3721 GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT
     3781 TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC
     3841 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
     3901 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
     3961 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
     4021 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
     4081 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
     4141 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
     4201 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
     4261 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
     4321 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
     4381 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT
     4441 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     4501 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     4561 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     4621 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     4681 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     4741 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     4801 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     4861 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     4921 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     4981 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     5041 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     5101 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     5161 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     5221 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     5281 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     5341 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     5401 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     5461 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     5521 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA
     5581 GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG
     5641 GAGTGGGAG
//