LOCUS       Exported     3445 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3445
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1482..1529
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3377..3393
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3394..3425
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1349
                     /label="AB_cI875/PL/PR"
                     /note=""
     primer_bind     complement(1534..1550)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1617..2205)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2396..3256)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3257..3361)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1385..1444
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAGGA GAAAAACCAT TCTTCATAAT TCAATCCATT
       61 TACTATGTTA TGTTCTGAGG GGAGTGAAAA TTCCCCTAAT TCGATGAAGA TTCTTGCTCA
      121 ATTGTTATCA GCTATGCGCC GACCAGAACA CCTTGCCGAT CAGCCAAACG TTTCTTCAGG
      181 CCACTGACTA GCGATAACTT TCCCCACAAC GGAACAACTC TCATTGCATG GGATCATTGG
      241 GTACTGTGGG TTTAGTGGTT GTAAAAACAC CTGACCGCTA TCCCTGATCA GTTTCTTGAA
      301 GGTAAACTCA TCACCCCCAA GTCTGGCTAT GCAGAAATCA CCTGGCTCAA CAGCCTGCTC
      361 AGGGTCAACG AGAATTAACA TTCCGTCAGG AAAGCTTGGC TTGGAGCCTG TTGGTGCGGT
      421 CATGGAATTA CCTTCAACCT CAAGCCAGAA TGCAGAATCA CTGGCTTTTT TGGTTGTGCT
      481 TACCCATCTC TCCGCATCAC CTTTGGTAAA GGTTCTAAGC TTAGGTGAGA ACATCCCTGC
      541 CTGAACATGA GAAAAAACAG GGTACTCATA CTCACTTCTA AGTGACGGCT GCATACTAAC
      601 CGCTTCATAC ATCTCGTAGA TTTCTCTGGC GATTGAAGGG CTAAATTCTT CAACGCTAAC
      661 TTTGAGAATT TTTGCAAGCA ATGCGGCGTT ATAAGCATTT AATGCATTGA TGCCATTAAA
      721 TAAAGCACCA ACGCCTGACT GCCCCATCCC CATCTTGTCT GCGACAGATT CCTGGGATAA
      781 GCCAAGTTCA TTTTTCTTTT TTTCATAAAT TGCTTTAAGG CGACGTGCGT CCTCAAGCTG
      841 CTCTTGTGTT AATGGTTTCT TTTTTGTGCT CATACGTTAA ATCTATCACC GCAAGGGATA
      901 AATATCTAAC ACCGTGCGTG TTGACTATTT TACCTCTGGC GGTGATAATG GTTGCATGTA
      961 CTAAGGAGGT TGTATGGAAC AACGCATAAC CCTGAAAGAT TATGCAATGC GCTTTGGGCA
     1021 AACCAAGACA GCTAAAGATC TCTCACCTAC CAAACAATGC CCCCCTGCAA AAAATAAATT
     1081 CATATAAAAA ACATACAGAT AACCATCTGC GGTGATAAAT TATCTCTGGC GGTGTTGACA
     1141 TAAATACCAC TGGCGGTGAT ACTGAGCACA TCAGCAGGAC GCACTGACCA CCATGAAGGT
     1201 GACGCTCTTA AAAATTAAGC CCTGAAGAAG GGCAGCATTC AAAGCAGAAG GCTTTGGGGT
     1261 GTGTGATACG AAACGAAGCA TTGGGATCTA TCGATGCATG CCATGGTACC CGGGAGCTCG
     1321 AATTAATTCT AGAAATAATT TTGTTTAACT TTTACTCGAG ACCAGGCCTT TTATTGGGTC
     1381 CAGTGCAGAG GCCACATTGG GCCAGTAGGG ATAACAGGGT AATGATACAA TGCCCCCGCA
     1441 ACTGCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG
     1501 CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC
     1561 AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC
     1621 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
     1681 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
     1741 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
     1801 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
     1861 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
     1921 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
     1981 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
     2041 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
     2101 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
     2161 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA
     2221 GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
     2281 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
     2341 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
     2401 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
     2461 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
     2521 CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
     2581 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
     2641 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
     2701 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
     2761 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
     2821 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
     2881 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
     2941 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
     3001 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
     3061 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
     3121 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
     3181 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
     3241 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
     3301 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
     3361 GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT
     3421 TGACTTGGTT CAGGTGGAGT GGGAG
//