LOCUS Exported 2405 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2405 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 442..489 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2337..2353 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2354..2385 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 99..239 /label="N1N2_Calm" /note="" primer_bind complement(494..510) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(577..1165) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1356..2216) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2217..2321) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 345..404 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AACGATGATG CTCACTCTCG AAGATTACTT TTTGGATACC TGAAACAAAT CCCATGGTAG 61 TCCTTAGACT TGGGAGTCTA TCACCTCTAG GGTCTCACCA TGGGCGTGAT GCCGCGCGAA 121 GAAACCGATA GCAAAACCGC GAGCCCGTGG AAAAGCGCGC GCCTGATGGT GCATACCGTG 181 GCGACCTTTA ACAGCATTAA AGAACTGAAC GAACGCTGGC GCAGCCTGCA GCAGCTGGCG 241 GGGTCACGAG ACCCTGGTAC AACAGGACTA TGAAACCGGT ACAAAGGCAA CATCCTCACT 301 TAGGTGAATC GAAACGCGGT ATCACCAGTA TCGGCAGCTA ATCTAGTGCC AGCGGTAGGA 361 TGGCCTAGGG ATAACAGGGT AATATGTATG ATTTTCATGT AGCACGCTGA GGTGTCAATC 421 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG 481 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC 541 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA 601 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 661 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 721 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 781 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 841 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 901 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 961 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 1021 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 1081 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 1141 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC 1201 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 1261 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 1321 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 1381 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 1441 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA 1501 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 1561 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 1621 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 1681 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 1741 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 1801 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 1861 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 1921 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 1981 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 2041 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 2101 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 2161 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 2221 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 2281 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA 2341 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT 2401 GGGAG //