LOCUS       Exported     3699 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3699
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1736..1783
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3631..3647
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3648..3679
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1603
                     /label="N1N2_CBD"
                     /note=""
     primer_bind     complement(1788..1804)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1871..2459)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2650..3510)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3511..3615)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1639..1698
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCACCA TGAAGATCGA AGAAGGGAAG TTAGTGATCG
       61 GATCGTTAGA AGGCTGCTTT GCGAAAGGAA CCAATGTCCT GATGGCGGAC GGTAGCATTG
      121 AATGTATCGA AAACATCGAA GTCGGAAACA AGGTGATGGG GAAAGATGGT CGTCCGCGTG
      181 AAGTTATCAA ACTGCCCCGT GGACGCGAAA CTATGTACTC AGTTGTCCAA AAGTCCCAGC
      241 ACCGTGCGCA CAAAAGTGAT TCGTCGCGTG AAGTGCCCGA GCTGTTGAAG TTCACCTGTA
      301 ACGCAACGCA CGAACTGGTA GTGCGTACAC CTCGCTCTGT GCGTCGTCTG TCTCGTACCA
      361 TTAAGGGTGT CGAGTATTTC GAGGTGATCA CTTTCGAAAT GGGTCAGAAG AAGGCACCCG
      421 ACGGTCGTAT TGTGGAACTG GTGAAAGAGG TGTCAAAGTC ATACCCGATC AGCGAAGGTC
      481 CGGAACGTGC CAATGAGTTA GTTGAATCGT ACCGCAAAGC TTCTAACAAA GCATACTTCG
      541 AATGGACCAT CGAAGCGCGT GACCTGTCTC TGCTGGGGAG CCATGTGCGT AAAGCAACCT
      601 ATCAGACCTA CGCTCCGATC CTGTACGAGA ACGACCACTT CTTTGATTAC ATGCAGAAAT
      661 CGAAATTCCA CCTGACCATC GAGGGGCCAA AAGTCCTGGC CTACTTACTG GGTCTTTGGA
      721 TTGGTGATGG TCTTAGCGAT CGTGCAACCT TCTCAGTAGA TAGTCGTGAT ACCTCTCTGA
      781 TGGAACGTGT GACTGAGTAT GCGGAAAAGC TGAATCTGTG TGCCGAGTAC AAGGACCGTA
      841 AGGAACCGCA AGTCGCGAAA ACCGTTAACC TGTATTCAAA AGTTGTTCGT GGTGCATCTA
      901 CGAACCCGGG AGTTAGTGCG TGGCAAGTCA ACACCGCTTA TACGGCTGGC CAACTGGTAA
      961 CCTATAACGG GAAAACGTAC AAGTGCCTGC AACCGCACAC CTCCTTGGCG GGATGGGAGC
     1021 CGAGCAACGT GCCAGCGCTG TGGCAATTGC AGGGCGGTCA CGGCGGTATC CGCAACAATT
     1081 TGAATACGGA AAACCCCTTG TGGGACGCGA TCGTGGGCCT GGGTTTCCTG AAAGACGGGG
     1141 TCAAGAACAT CCCATCCTTC CTGAGCACCG ATAACATTGG TACGCGTGAG ACATTCCTGG
     1201 CAGGATTGAT TGATTCGGAT GGCTATGTGA CTGACGAACA CGGTATTAAG GCTACTATCA
     1261 AAACCATTCA TACCTCTGTT CGCGATGGGC TGGTTTCTTT AGCTCGCTCG CTGGGTTTAG
     1321 TGGTGAGCGT TAACGCGGAA CCGGCAAAAG TCGATATGAA TGTGACCAAA CACAAGATCT
     1381 CTTATGCGAT TTACATGTCT GGCGGAGATG TTCTGCTTAA CGTTCTGTCT AAATGTGCGG
     1441 GTAGCAAGAA ATTCCGTCCT GCGCCAGCGG CAGCTTTTGC GCGTGAATGT CGTGGTTTCT
     1501 ACTTCGAATT GCAAGAATTG AAAGAGGACG ACTATTACGG CATTACCCTG TCCGACGACA
     1561 GCGATCATCA ATTCCTGTTG GGTTCTCAAG TGGTCGTACA AAACGGGTCA CGAGACCAGT
     1621 CCGTCGGAGT AACAATCTTT CATGAAGCTA CACAAGCGCT AGGGATAACA GGGTAATTGA
     1681 TCTCAGGGCC TCTAAACACG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC
     1741 ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT
     1801 CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA
     1861 ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
     1921 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
     1981 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
     2041 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
     2101 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
     2161 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
     2221 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
     2281 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA
     2341 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
     2401 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
     2461 AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
     2521 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
     2581 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
     2641 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG
     2701 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
     2761 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC
     2821 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC
     2881 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
     2941 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT
     3001 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG
     3061 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT
     3121 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG
     3181 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
     3241 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT
     3301 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT
     3361 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC
     3421 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT
     3481 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     3541 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     3601 AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC
     3661 CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//