LOCUS       Exported     2418 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2418
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      455..502
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2350..2366
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2367..2398
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             159..192
                     /label="N1N3_GS flexible"
                     /note=""
     primer_bind     complement(507..523)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(590..1178)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1369..2229)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2230..2334)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    358..417
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG TAATCCTCAT CAATTATAAA ATATGAGGTA AGCCAAAAAA
       61 GCACGTGGTG GCGCTCACCG ACTGTTCCCA AACTGTAACT CATTGTTCTG TCAAGGCCTG
      121 ACTTACTTCC CGGCCCTTTC CATGTGCGGA GGTCTCACCA TGGGAGGTGG CTCTGGCGGT
      181 GGATCAGGTG GAGGCTCCAC GAGACCGCAG ATAGATTGCC AAGCATAGAG CTTACGAGCC
      241 AGCGAAAACA GTAAGGCCTT TTTAAGTATG GGGAGTAAGT GATCGAACGC TTCAGATATG
      301 ACTATATACT TAGGTTAGAT CTCGTCCCGT GAATTTAGCT GGGCACTATG CTTTACTTCG
      361 CGCGGGTCGA GCGCCCCATA GGGATAACAG GGTAATCGGC TCTCCCACTA AACCTGACGC
      421 TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA
      481 ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT
      541 GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC
      601 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
      661 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
      721 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
      781 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
      841 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
      901 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
      961 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     1021 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA
     1081 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
     1141 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA
     1201 CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     1261 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     1321 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     1381 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     1441 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     1501 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     1561 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     1621 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     1681 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     1741 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     1801 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     1861 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     1921 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     1981 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     2041 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     2101 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     2161 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     2221 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     2281 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     2341 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     2401 GTTCAGGTGG AGTGGGAG
//