LOCUS       Exported     2997 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2997
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1034..1081
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2929..2945
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2946..2977
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..901
                     /label="BC_BCD2_His_fuGFP_TEVcut"
                     /note=""
     primer_bind     complement(1086..1102)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1169..1757)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1948..2808)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2809..2913)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    937..996
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCATAC TGGGCCCAAG TTCACTTAAA AAGGAGATCA
       61 ACAATGAAAG CAATTTTCGT ACTGAAACAT CTTAATCATG CTAAGGAGGT TTTCTAATGG
      121 GCAGCAGCCA TCACCATCAC CATCACAGCA GCGGCGTTTC TAGCGGTGAA GATATCTTCT
      181 CAGGCCTGGT CCCGATTCTG ATCGAGCTGG AGGGCGATGT GAATGGCCAC CGCTTCTCTG
      241 TTCGTGGTGA AGGTTATGGC GACGCCTCTA ACGGCAAACT GGAAATTAAG TTCATCTGCA
      301 CCACCGGTCG TCTGCCGGTT CCGTGGCCGA CGCTGGTGAC TACTCTGTCT TACGGTGTCC
      361 AGTGCTTCGC AAAATACCCG GAACACATGC GTCAGAATGA TTTTTTCAAG TCCGCCATGC
      421 CAGATGGCTA TGTTCAGGAA CGTACCATCA GCTTCAAAGA GGACGGTACG TACAAAACCC
      481 GTGCGGAAGT TAAATTTGAA GGTGAAGCGC TGGTGAACCG TATCGACTTA AAAGGTCTGG
      541 AGTTCAAGGA AGATGGCAAC ATTCTGGGCC ACAAACTGGA ATACAGCTTC AACAGCCACT
      601 ATGTTTATAT TACCGCGGAC AAAAATCGCA ACGGCCTGGA GGCGCAGTTC CGTATCCGTC
      661 ACAACGTCGA CGACGGTTCT GTCCAGCTTG CGGACCATTA CCAGCAGAAC ACCCCGATCG
      721 GTGAGGGCCC AGTACTGCTC CCGGAGCAAC ACTACCTGAC GACTAACTCT GTCCTGTCCA
      781 AAGACCCGCA GGAACGCCGT GATCACATGG TTCTGGTTGA ATTTGTGACC GCTGCCGGCC
      841 TGTCGCTGGG CATGGACGAA CTGTATAAAT CCAGCGGCGG AGAAAATCTT TATTTTCAAG
      901 GTGGAATGCG AGACCACCGC GTGGTTCTTA CAGTATGCTG CGCCACACGC TACTGGTTAG
      961 GGATAACAGG GTAATCCCCG AGCATTTGCG CGTGTACGCT GAGGTGTCAA TCGTCGGAGC
     1021 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT
     1081 GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG
     1141 TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC
     1201 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
     1261 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
     1321 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
     1381 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
     1441 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
     1501 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
     1561 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG
     1621 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
     1681 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     1741 GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC
     1801 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     1861 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     1921 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     1981 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     2041 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC
     2101 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     2161 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     2221 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     2281 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     2341 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     2401 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     2461 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     2521 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     2581 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     2641 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     2701 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     2761 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     2821 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     2881 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG
     2941 CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAG
//