LOCUS       Exported     2856 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2856
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      893..940
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2788..2804
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2805..2836
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..760
                     /label="N2N3_fuGFP"
                     /note=""
     primer_bind     complement(945..961)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1028..1616)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1807..2667)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2668..2772)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    796..855
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAGTC AGTTTCTAGC GGTGAAGATA TCTTCTCAGG
       61 CCTGGTCCCG ATTCTGATCG AGCTGGAGGG CGATGTGAAT GGCCACCGCT TCTCTGTTCG
      121 TGGTGAAGGT TATGGCGACG CCTCTAACGG CAAACTGGAA ATTAAGTTCA TCTGCACCAC
      181 CGGTCGTCTG CCGGTTCCGT GGCCGACGCT GGTGACTACT CTGTCTTACG GTGTCCAGTG
      241 CTTCGCAAAA TACCCGGAAC ACATGCGTCA GAATGATTTT TTCAAGTCCG CCATGCCAGA
      301 TGGCTATGTT CAGGAACGTA CCATCAGCTT CAAAGAGGAC GGTACGTACA AAACCCGTGC
      361 GGAAGTTAAA TTTGAAGGTG AAGCGCTGGT GAACCGTATC GACTTAAAAG GTCTGGAGTT
      421 CAAGGAAGAT GGCAACATTC TGGGCCACAA ACTGGAATAC AGCTTCAACA GCCACTATGT
      481 TTATATTACC GCGGACAAAA ATCGCAACGG CCTGGAGGCG CAGTTCCGTA TCCGTCACAA
      541 CGTCGACGAC GGTTCTGTCC AGCTTGCGGA CCATTACCAG CAGAACACCC CGATCGGTGA
      601 GGGCCCAGTA CTGCTCCCGG AGCAACACTA CCTGACGACT AACTCTGTCC TGTCCAAAGA
      661 CCCGCAGGAA CGCCGTGATC ACATGGTTCT GGTTGAATTT GTGACCGCTG CCGGCCTGTC
      721 GCTGGGCATG GACGAACTGT ATAAATCCGG CGGTGGCGGT GGATCCACGA GACCAGACCC
      781 GTCCTTTGTT GTGATGTAAA AGTGACATCG ACACTCTAGG GATAACAGGG TAATAGTCCG
      841 CCAACCGCCA CTGAGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA
      901 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT
      961 GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG
     1021 CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
     1081 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
     1141 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
     1201 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
     1261 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
     1321 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
     1381 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
     1441 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
     1501 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
     1561 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     1621 GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     1681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     1741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1801 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     1861 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     1921 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     1981 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     2041 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     2101 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     2161 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     2221 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     2281 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     2341 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     2401 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     2461 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     2521 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     2581 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     2641 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     2701 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     2761 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     2821 CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//