LOCUS       Exported     2796 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2796
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      833..880
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2728..2744
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2745..2776
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..700
                     /label="N2N3_AmilCP"
                     /note=""
     primer_bind     complement(885..901)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(968..1556)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1747..2607)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2608..2712)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    736..795
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAGTC AGTAATTGCG AAACAAATGA CATATAAAGT
       61 TTATATGTCA GGTACGGTTA ATGGTCATTA CTTCGAAGTT GAAGGTGACG GTAAGGGTAA
      121 ACCATACGAG GGTGAACAAA CTGTTAAATT AACAGTAACA AAAGGTGGCC CTTTACCATT
      181 TGCTTGGGAT ATCTTATCTC CACAATGTCA ATACGGTTCA ATCCCATTTA CTAAGTATCC
      241 AGAAGATATT CCGGACTATG TAAAACAGTC TTTTCCTGAA GGTTACACTT GGGAACGTAT
      301 TATGAACTTC GAGGACGGTG CAGTATGTAC GGTATCTAAT GATTCTTCTA TTCAGGGTAA
      361 CTGTTTCATT TATCATGTAA AATTTTCTGG TTTAAATTTT CCACCAAACG GGCCAGTAAT
      421 GCAAAAAAAA ACACAAGGTT GGGAACCAAA TACTGAACGT TTATTCGCAC GTGATGGTAT
      481 GCTTTTAGGT AACAACTTTA TGGCGTTGAA ATTAGAGGGC GGTGGTCATT ACTTGTGTGA
      541 GTTCAAGACA ACTTATAAAG CTAAAAAACC GGTAAAAATG CCAGGTTACC ACTACGTAGA
      601 TCGTAAATTA GATGTTACGA ACCATAACAA AGACTATACT TCTGTAGAAC AATGCGAAAT
      661 TAGTATTGCT CGCAAACCAG TAGTTGCCGG CGGTGGCGGT GGATCCACGA GACCACAGCC
      721 TCTGTGTTGT CTGATGTCCT AAAACATAAA GACGACTAGG GATAACAGGG TAATTCGCTA
      781 TTTATTGTCA TCGTCCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA
      841 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT
      901 GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG
      961 CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
     1021 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
     1081 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
     1141 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
     1201 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
     1261 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
     1321 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
     1381 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
     1441 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
     1501 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     1561 GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     1621 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     1681 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1741 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     1801 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     1861 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     1921 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     1981 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     2041 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     2101 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     2161 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     2221 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     2281 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     2341 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     2401 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     2461 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     2521 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     2581 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     2641 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     2701 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     2761 CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//