LOCUS       Exported     4106 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4106
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      2143..2190
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4038..4054
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4055..4086
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2010
                     /label="CD_OMMLV"
                     /note=""
     primer_bind     complement(2195..2211)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2278..2866)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3057..3917)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3918..4022)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2046..2105
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GACTCTGAAC ATCGAAGATG AACATCGTCT
       61 GCACGAAACC AGCAAAGAAC CGGACGTTTC TCTGGGTTCA ACCTGGCTGT CTGATTTTCC
      121 GCAAGCTTGG GCCGAAACTG GCGGTATGGG CCTGGCCGTC CGTCAGGCAC CGCTGATCAT
      181 CCCACTGAAA GCGACCTCCA CCCCGGTGTC CATCAAACAG TATCCAATGT CCCAGGAAGC
      241 ACGTCTGGGC ATCAAGCCAC ATATCCAACG TCTGCTGGAT GCCGGTATCC TGGTACCGTG
      301 TCAATCGCCT TGGAACACTC CACTGCTGCC GGTGAAAAAA CCGGGCACTA ACGATTACCG
      361 TCCGGTACAG GACCTGCGTG AAGTTAACAA ACGTGTTGAA GATATTCATC CGACCGTCCC
      421 GAATCCGTAC AACCTCCTGT CCGGTCTGCC GCCGAGCCAT CAGTGGTACA CCGTGCTGGA
      481 CCTGAAGGAC GCATTCTTTT GCCTGCGTCT GCACCCGACC TCTCAGCCGC TGTTTGCGTT
      541 TGAATGGCGT GACCCGGAAA TGGGTATTTC TGGCCAGCTT ACATGGACCC GCCTGCCACA
      601 GGGTTTCAAA AACTCCCCGA CCCTTTTCGA CGAAGCCCTG CACCGTGACC TGGCAGATTT
      661 TCGCATTCAG CACCCGGACC TCATCCTGCT GCAATACATG GACGATCTGC TGTTGGCAGC
      721 TACCAGCGAA CTGGATTGCC AGCAGGGTAC CCGTGCGCTG CTGCAAACCC TGGGCAACCT
      781 GGGTTACCGT GCTTCCGCGA AAAAGGCGCA GATTTGCCAA AAACAGGTCA AATATCTGGG
      841 TTACCTGCTG AAAGAAGGCC AGCGCTGGCT GACCGAGGCC CGCAAAGAAA CCGTGATGGG
      901 TCAACCGACC CCGAAAACCC CGCGTCAACT GCGCGAGTTT CTGGGTACCG CTGGCTTCTG
      961 CCGTCTGTGG ATTCCGGGCT TCGCTGAAAT GGCCGCGCCG CTGTATCCGT TAACCAAAAC
     1021 TGGTACTCTG TTCAACTGGG GTCCGGACCA GCAGAAAGCC TATCAGGAGA TTAAACAAGC
     1081 GCTGCTGACC GCACCGGCGC TGGGCCTGCC GGACCTGACC AAACCATTTG AACTGTTCGT
     1141 TGACGAGAAA CAAGGTTACG CTAAAGGTGT TCTTACCCAG AAACTGGGTC CGTGGCGCCG
     1201 TCCGGTAGCG TATCTGTCTA AAAAACTGGA CCCGGTTGCG GCTGGTTGGC CGCCGTGTCT
     1261 GCGTATGGTT GCTGCTATTG CCGTACTGAC TAAGGATGCG GGCAAACTGA CTATGGGCCA
     1321 GCCGCTGGTG ATCCTGGCTC CGCATGCGGT GGAGGCCCTG GTTAAACAGC CGCCGGATCG
     1381 CTGGCTGTCT AACGCGCGTA TGACACACTA CCAGGCTCTG CTGCTGGATA CCGACCGTGT
     1441 TCAGTTCGGC CCGGTCGTGG CCCTGAACCC GGCGACCCTG CTCCCGCTGC CGGAAGAAGG
     1501 TCTGCAACAC AATTGCCTGG ATATTCTGGC GGAAGCCCAT GGTACTCGTC CGGATCTGAC
     1561 CGACCAGCCT CTGCCGGATG CAGACCACAC CTGGTACACT GGTGGCAGCT CCCTCCTGCA
     1621 AGAGGGCCAG CGTAAGGCAG GCGCGGCTGT TACCACGGAA ACCGAAGTAA TCTGGGCTAA
     1681 AGCCCTGCCA GCAGGAACGT CAGCCCAACG CGCACAACTG ATTGCTCTGA CCCAGGCACT
     1741 GAAAATGGCG GAAGGTAAAA AGCTGAACGT TTACACTAAC TCCCGCTACG CATTCGCTAC
     1801 CGCCCACATC CATGGCGAAA TCTACAGACG TCGTGGTCTG CTGACCTCTG AAGGCAAAGA
     1861 AATCAAAAAC AAAGATGAGA TCCTGGCCCT GCTGAAAGCG CTGTTCCTGC CTAAACGTTT
     1921 ATCAATCATC CACTGTCCGG GCCACCAGAA GGGCCACTCC GCGGAAGCGC GCGGCAACCG
     1981 TATGGCAGAC CAGGCAGCAC GCAAAGCTGC CGGAGGTCGA GACCAGAGTC GTCCAACCCT
     2041 TGAATACGAA AATAGGGGCT GAAGATTAGG GATAACAGGG TAATGCATAA TCCGACCCCA
     2101 CTCGGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     2161 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     2221 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     2281 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     2341 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     2401 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     2461 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     2521 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     2581 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     2641 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     2701 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     2761 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     2821 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     2881 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     2941 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     3001 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     3061 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     3121 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     3181 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     3241 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     3301 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     3361 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     3421 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     3481 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     3541 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     3601 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     3661 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     3721 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     3781 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     3841 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     3901 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     3961 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     4021 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     4081 TTGACTTGGT TCAGGTGGAG TGGGAG
//