LOCUS       Exported     3821 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3821
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1858..1905
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3753..3769
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3770..3801
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1725
                     /label="CD_HIVRT"
                     /note=""
     primer_bind     complement(1910..1926)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1993..2581)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2772..3632)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3633..3737)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1761..1820
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GGGCGGTAGT GGCATGCCGA TCTCGCCGAT
       61 TGAAACCGTG CCCGTGAAAT TAAAACCGGG CATGGATGGG CCGAAAGTCA AACAGTGGCC
      121 ACTGACCGAA GAGAAGATTA AAGCGTTAGT GGAGATTTGT ACCGAAATGG AAAAGGAGGG
      181 GAAGATCAGC AAGATCGGGC CAGAGAATCC GTACAATACC CCAGTTTTTG CGATTAAAAA
      241 GAAAGATAGT ACGAAGTGGC GCAAATTGGT CGATTTTCGT GAGCTCAATA AACGTACGCA
      301 GGATTTCTGG GAAGTGCAGC TGGGCATACC GCATCCGGCG GGCCTGAAGA AAAAAAAATC
      361 AGTGACGGTC TTAGATGTTG GTGATGCGTA TTTTAGCGTT CCGCTGGATG AGGACTTTCG
      421 GAAATACACT GCATTTACGA TCCCTTCCAT TAATAACGAA ACGCCGGGCA TTAGGTATCA
      481 GTACAATGTA CTTCCGCAGG GTTGGAAAGG GAGCCCGGCA ATCTTTCAGA GTTCTATGAC
      541 CAAAATCCTG GAACCGTTTC GTAAGCAAAA CCCGGATATT GTAATTTATC AGTACATGGA
      601 CGATTTGTAT GTCGGCTCTG ACCTCGAAAT AGGTCAGCAT CGTACAAAAA TTGAAGAACT
      661 GCGGCAGCAT TTACTCCGCT GGGGGCTGAC CACCCCGGAT AAAAAGCACC AGAAGGAACC
      721 TCCCTTCCTG TGGATGGGCT ACGAGCTGCA TCCTGATAAA TGGACTGTGC AACCAATCGT
      781 GCTGCCCGAG AAAGATTCGT GGACCGTGAA CGATATCCAG AAACTGGTCG GAAAGCTGAA
      841 TTGGGCCTCT CAAATCTATC CGGGTATTAA GGTACGACAA CTGTGCAAAC TGCTGCGCGG
      901 TACTAAGGCC TTGACTGAGG TAATCCCACT GACAGAAGAA GCGGAACTAG AATTGGCTGA
      961 GAACCGTGAA ATCTTAAAAG AGCCGGTGCA CGGTGTTTAT TATGACCCGA GCAAAGACCT
     1021 TATCGCGGAA ATACAGAAAC AGGGTCAGGG TCAATGGACG TATCAAATCT ATCAGGAACC
     1081 ATTCAAAAAC CTGAAAACAG GCAAATATGC ACGCATGCGT GGCGCGCACA CAAACGACGT
     1141 GAAACAACTT ACGGAAGCTG TGCAAAAAAT CACCACCGAA AGCATTGTGA TATGGGGAAA
     1201 AACCCCTAAG TTTAAACTGC CGATTCAAAA AGAAACTTGG GAAACCTGGT GGACAGAGTA
     1261 TTGGCAGGCG ACGTGGATTC CCGAATGGGA GTTCGTCAAC ACCCCCCCTC TTGTTAAACT
     1321 CTGGTACCAG CTAGAAAAAG AACCGATTGT TGGCGCAGAA ACCTTCTACG TAGATGGAGC
     1381 CGCAAATCGT GAAACTAAAC TTGGAAAAGC CGGCTATGTG ACGAACAGAG GTCGCCAGAA
     1441 AGTGGTCACC CTGACGGACA CAACTAATCA GAAAACGGAG CTTCAGGCCA TCTATTTGGC
     1501 CCTGCAAGAT TCCGGACTGG AAGTAAATAT TGTTACCGAT TCCCAATACG CACTCGGGAT
     1561 CATTCAGGCG CAGCCAGACC AATCGGAAAG CGAGCTGGTT AACCAGATTA TTGAACAGCT
     1621 GATTAAAAAG GAAAAAGTCT ACTTAGCTTG GGTTCCTGCG CATAAAGGTA TCGGTGGTAA
     1681 CGAACAAGTA GATAAACTGG TGTCAGCTGG CATTCGCAAA GTTCTGGGAG GTCGAGACCA
     1741 CCATCTTGCG TCGTGAGAAT AAGGGCAGGA TACCTAGGCA TTAGGGATAA CAGGGTAATT
     1801 GAGTCTATAA TATTATGTAA CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1861 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1921 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1981 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     2041 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     2101 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     2161 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     2221 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     2281 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     2341 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     2401 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     2461 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2521 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2581 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2641 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2701 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2761 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2821 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2881 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2941 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     3001 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     3061 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     3121 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     3181 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     3241 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     3301 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     3361 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     3421 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     3481 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     3541 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     3601 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3661 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3721 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3781 TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA G
//