LOCUS       Exported     3890 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3890
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1927..1974
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3822..3838
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3839..3870
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1794
                     /label="CD_Bst-LF"
                     /note=""
     primer_bind     complement(1979..1995)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2062..2650)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2841..3701)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3702..3806)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1830..1889
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GACCGACGAA GGCGAAAAAC CGCTGGCGGG
       61 TATGGACTTT GCGATCGCTG ATAGCGTTAC CGACGAGATG CTGGCAGATA AAGCAGCGCT
      121 AGTAGTTGAA GTTGTGGGCG ATAACTACCA TCATGCGCCG ATCGTGGGTA TCGCTTTGGC
      181 GAACGAGCGC GGCCGTTTTT TCCTGCGCCC GGAAACCGCA CTGGCGGACC CGAAGTTTCT
      241 GGCTTGGCTG GGCGACGAAA CGAAAAAAAA GACTATGTTC GATTCTAAAC GTGCAGCGGT
      301 GGCTCTCAAA TGGAAAGGTA TCGAATTGCG CGGTGTTGTG TTCGACCTGC TGCTGGCTGC
      361 GTACCTGCTC GATCCTGCTC AGGCTGCGGG TGACGTGGCA GCTGTAGCGA AAATGCACCA
      421 GTACGAAGCC GTACGCTCTG ATGAAGCGGT TTATGGTAAA GGTGCGAAAC GTACCGTGCC
      481 AGACGAACCA ACCCTGGCGG AACACCTGGC TCGCAAAGCA GCCGCTATCT GGGCCCTGGA
      541 GGAGCCTCTG ATGGATGAAC TTCGCCGCAA CGAACAGGAC CGTCTGCTCA CAGAACTGGA
      601 ACAGCCGTTG GCGGGTATTC TGGCAAACAT GGAGTTCACT GGTGTAAAAG TGGACACTAA
      661 GCGCCTGGAA CAAATGGGTG CCGAACTGAC TGAGCAGCTT CAGGCAGTTG AACGCCGCAT
      721 CTACGAACTG GCGGGTCAGG AGTTTAACAT CAACTCTCCT AAACAGCTGG GCACCGTGTT
      781 GTTCGACAAA CTGCAACTGC CGGTGTTGAA GAAAACGAAA ACCGGCTACA GCACCTCCGC
      841 CGATGTTCTG GAAAAACTGG CACCGCATCA CGAAATCGTT GAACACATCC TGCACTACCG
      901 TCAGCTGGGT AAACTGCAAT CTACCTACAT CGAGGGCTTA CTGAAAGTAG TGCACCCGGT
      961 GACCGGTAAA GTGCACACTA TGTTCAACCA GGCTCTGACG CAGACCGGCC GCCTGTCCTC
     1021 CGTTGAACCG AACCTGCAAA ACATCCCCAT CCGTCTGGAA GAGGGCCGTA AGATCCGTCA
     1081 GGCATTCGTG CCGTCTGAAC CGGACTGGCT GATTTTCGCT GCTGATTACT CTCAAATCGA
     1141 ACTGCGTGTT CTTGCTCACA TTGCGGAGGA CGATAACCTG ATTGAAGCAT TCCGTCGTGG
     1201 TCTGGACATT CACACTAAAA CGGCTATGGA CATTTTCCAT GTTTCCGAAG AAGATGTGAC
     1261 CGCTAATATG CGTCGTCAGG CTAAAGCGGT AAACTTTGGT ATCGTATACG GTATCTCCGA
     1321 TTACGGCCTG GCACAGAACC TGAACATCAC CCGCAAAGAA GCGGCTGAGT TTATCGAACG
     1381 TTATTTCGCC TCGTTCCCCG GTGTTAAACA GTACATGGAT AACATCGTTC AAGAAGCTAA
     1441 ACAGAAGGGT TATGTGACCA CCCTGCTGCA CCGTCGTCGT TACCTGCCGG ACATCACCTC
     1501 CCGTAACTTC AACGTGCGTT CTTTCGCCGA ACGTACGGCT ATGAATACCC CTATCCAGGG
     1561 CAGCGCGGCG GACATCATCA AAAAAGCCAT GATCGACCTG AGCGTGCGCC TGCGCGAAGA
     1621 ACGTTTGCAG GCTCGCCTGC TTCTGCAAGT GCACGATGAG CTGATCCTAG AAGCTCCAAA
     1681 AGAGGAAATT GAACGTTTGT GCCGTCTGGT TCCGGAAGTT ATGGAACAGG CTGTCGCACT
     1741 GCGTGTTCCG CTTAAAGTTG ATTACCATTA CGGTCCCACT TGGTACGACG CCAAGGGAGG
     1801 TCGAGACCAC GAGTGTAGTT CCCGTCAATC CCGTCTACTA TTTTGCGGTC TAGGGATAAC
     1861 AGGGTAATGC CGAAGATCGC GTACGTGGCC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1921 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1981 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     2041 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     2101 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     2161 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     2221 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     2281 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     2341 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     2401 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     2461 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     2521 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     2581 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     2641 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     2701 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     2761 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     2821 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2881 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2941 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     3001 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     3061 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     3121 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     3181 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     3241 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     3301 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     3361 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     3421 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     3481 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     3541 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     3601 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     3661 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     3721 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3781 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3841 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
//